Expression of fungal desaturase genes in cultured mammalian cells

Molecular and Cellular Biochemistry

Volumn 219, Issue 1-2, 2001, Pages 7-11

  Kelder, Bruce ^a   Mukerji, Pradip ^b   Kirchner, Stephen ^b   Hovanec, Gregory ^a   Leonard, Amanda E ^b   Chuang, Lu Te ^b   Kopchick, John J ^b   Huang, Yung Sheng ^b  

^a OHIO UNIVERSITY   (United States)

^b ABBOTT LABORATORIES   (United States)

Author keywords

12 desaturase; 6 desaturase; cDNA; Essential fatty acids; Mouse L cells

Indexed keywords

ACYL COENZYME A DESATURASE; ARACHIDONIC ACID; DIHOMO GAMMA LINOLENIC ACID; ESSENTIAL FATTY ACID; FATTY ACID; FUNGAL ENZYME; LINOLEIC ACID; LINOLENIC ACID; OLEIC ACID; PHOSPHATIDYLCHOLINE; PHOSPHOLIPID; POLYUNSATURATED FATTY ACID;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL FUNCTION; CELL STRUCTURE; CONTROLLED STUDY; CULTURE MEDIUM; DIET; DNA MODIFICATION; ENZYME ACTIVITY; FATTY ACID METABOLISM; FATTY ACID SYNTHESIS; GENE EXPRESSION; INCUBATION TIME; L CELL; MAMMAL CELL; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT; PROTEIN EXPRESSION;

ALPHA-LINOLENIC ACID; ANIMALS; CLONING, MOLECULAR; CYTOMEGALOVIRUS; FATTY ACID DESATURASES; FATTY ACIDS, ESSENTIAL; FATTY ACIDS, UNSATURATED; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENES, FUNGAL; HUMANS; L CELLS (CELL LINE); LINOLEIC ACID; MICE; MORTIERELLA; OLEIC ACID; PLASMIDS; RECOMBINATION, GENETIC; TIME FACTORS; TRANSFECTION; TRANSFORMATION, GENETIC;

ANIMALIA; FUNGI; MAMMALIA;

EID: 0035046698     PISSN: 03008177     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1011023632564     Document Type: Article

References (13)

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3. Membrane lipid composition and cellular function
4. Diet fat composition alters membrane phospholipid composition, insulin binding, and glucose metabolism in adipocytes from control and diabetic animals
5. Essential fatty acids in human nutrition
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7. A comparison of bovine growth-hormone gene expression in mouse L cells directed by the molony murine-leukemia virus long terminal repeat, Simian virus-40 early promoter or Cytomegalovirus immediate-early promoter
8. Remodeling of mouse milk glycoconjugates by transgenic expression of a human glycosyltransferase
9. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA donor
10. Identification of Δ5-desaturase from Mortierella alpina by heterologous expression in baker's yeast and canola
11. Biochemistry of essential fatty acids
12. Nutritional and hormonal factors influencing desaturation of essential fatty acids