TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections

Histochemistry and Cell Biology

Volumn 115, Issue 4, 2001, Pages 293-299

  Bink, Karin ^a,b   Walch, Axel ^b   Feuchtinger, Annette ^a   Eisenmann, Heinrich ^a   Hutzler, Peter ^a   Höfler, Heinz ^a,b   Werner, Martin ^b  

^a INSTITUTE OF EPIDEMIOLOGY   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

3 D fluorescence spectra; Bleaching; Confocal laser scanning microscope (CLSM); Fluorescence in situ hybridisation (FISH); Nuclear staining

Indexed keywords

BO PRO 1; CYANINE DYE; DIMER; FLUORESCENT DYE; MONOMER; NEU DIFFERENTIATION FACTOR; NUCLEIC ACID; PARAFFIN; PROPIDIUM IODIDE; SYTOX GREEN; TO PRO 3; UNCLASSIFIED DRUG; YO PRO 3; YOYO 1;

ARGON LASER; ARTICLE; BLEACHING; BREAST CARCINOMA; CELL NUCLEUS; CENTROMERE; CONFOCAL LASER MICROSCOPY; CYTOPLASM; FEMALE; FLUORESCENCE; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; HELIUM NEON LASER; HUMAN; HUMAN TISSUE; ONCOGENE NEU; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING; TISSUE SECTION;

EID: 0035046196     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004180100254     Document Type: Article

References (16)

1. Comparative FISH analyses of numerical chromosome 7 abnormalities in 5-μm and 15-μm paraffin-embedded tissue sections from prostatic carcinoma
2. Fluorochrome-labeled RNA as a sensitive, strand-specific probe for direct fluorescence in situ hybridization
3. Direct visulization of individual DNA molecules by fluorescence microscopy: Characterization of the factors affecting signal/background and optimation of imaging conditions using YOYO
4. Use of nucleic acid dyes Syto-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry
5. TOTO and YOYO: New very bright fluorochromes for DNA content analyses by flow cytometry
6. Multiplex FISH and three-dimensional DNA imaging with near infrared femtosecond laser pulses
7. Three-dimensional reconstruction by confocal laser scanning microscopy in routine pathologic specimens of benign and malignant lesions of the human breast
8. Detection of human papilloma-virus DNA in CaSki and HeLa cells by fluorescence in situ hybridisation. Analyses by flow cytometry and confocal laser scanning microscopy
9. Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1, and PicoGreen for flow cytometric analyses of marine prokaryontes
10. Nuclear staining for laser confocal microscopy
11. A method for staining of cell nuclei in Xenopus laevis embryos with cyanine dyes for whole-mount confocal laser scanning microscopy
12. Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: Properties and applications
13. Detection and amplification systems for sensitive, multiple-target DNA and RNA in situ hybridization: Looking inside cells with a spectrum of colors
14. DNA staining for fluorescence and laser confocal microscopy
15. Highly sensitive, specific, and stable new fluorescent DNA stains for confocal laser microscopy and image processing of normal paraffin sections
16. Interphase cytogenetics in pathology: Principles, methods, an application of fluorescence in situ hybridization (FISH)