Adipogenic potential of human adipose derived stromal cells from multiple donors is heterogeneous

Journal of Cellular Biochemistry

Volumn 81, Issue 2, 2001, Pages 312-319

  Sen, Anindita ^a   Lea Currie, Y Renee ^a   Sujkowska, Danuta ^a   Franklin, Dawn M ^a   Wilkison, William O ^a,b   Halvorsen, Yuan Di C ^a,b   Gimble, Jeffrey M ^a,b  

^a Zen Bio Inc   (United States)

^b Artecel Sciences Inc   (United States)

Author keywords

Adipose; aP2; Branched DNA; Heterogeneous; Stromal cells

Indexed keywords

4 THIAZOLIDINONE DERIVATIVE; FATTY ACID BINDING PROTEIN; GENE PRODUCT; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; LEPTIN; MESSENGER RNA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR;

ADIPOCYTE; ADULT; ARTICLE; CELL DIFFERENTIATION; CELL HETEROGENEITY; CONTROLLED STUDY; FEMALE; GENE EXPRESSION; GENE INDUCTION; HUMAN; HUMAN CELL; HUMAN TISSUE; LIPID STORAGE; LIPOGENESIS; MALE; OBESITY; PRIORITY JOURNAL; PROADIPOCYTE; PROTEIN SECRETION; STROMA CELL;

ADIPOCYTES; ADULT; AZO COMPOUNDS; CARRIER PROTEINS; CELL DIFFERENTIATION; CELL DIVISION; DNA; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME-LINKED IMMUNOSORBENT ASSAY; FATTY ACID-BINDING PROTEINS; FLUORESCENT DYES; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; LEPTIN; NEOPLASM PROTEINS; OBESITY; OXAZINES; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TUMOR SUPPRESSOR PROTEINS;

EID: 0035043970     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-4644(20010501)81:2<312::AID-JCB1046>3.0.CO;2-Q     Document Type: Article

References (33)

1. Regulation of adipose cell differentiation. I. Fatty acids are inducers of the aP2 gene expression
2. Expression of specific mRNAs during adipose differentiation: Identification of an mRNA encoding a homologue of myelin P2 protein
3. Evidence for an increase in transcription of specific mRNAs during differentiation of 3T3-L1 preadipocytes
4. A novel method for analysis of nuclear receptor function at natural promoters: Peroxisome proliferator-activated receptor gamma agonist actions on aP2 gene expression detected using branched DNA messenger RNA quantitation
5. Single step method of RNA isolation by acid guanidium-isothiocyanate phenolchloroform extraction
6. Differentiation of human adipocyte precursors in a chemically defined serum-free medium
7. Osteoblastic gene expression during adipogenesis in hematopoietic supporting murine bone marrow stromal cells
8. Identification of a potent adipocyte-specific enhancer: Involvement of an NF-1-like factor
9. Identification of a fat cell enhancer: Analysis of requirements for adipose tissue-specific gene expression
10. Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression
11. An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion
12. Understanding adipocyte differentiation
13. Localization of a pioglitazone response element in the adipocyte fatty acid-binding protein gene
14. Quantitation of HBV DNA in human serum using a branched DNA (bDNA) signal amplification assay
15. Adipocyte P2 gene: Developmental expression and homology of 5′-flanking sequences among fat cell-specific genes
16. Adipocyte fatty acid-binding protein: Regulation of gene expression in vivo and in vitro by an insulin-sensitizing agent
17. Control of rat preadipocyte adipose conversion by ovarian status: Regional specificity and possible involvement of the mitogen-activated protein-kinase dependent and c-fos signaling pathways
18. Site related specificities of the control by androgenic status of adipogenesis and mitogen-activated protein-kinase cascade/c-fos signaling pathways in rat preadipocytes
19. An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor gamma (PPAR gamma)
20. Human obese gene expression: Adipocyte specific expression and regional differences in the adipose tissue
21. Depot-related gene expression in human subcutaneous and omental adipocytes
22. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction
23. Branched DNA signal amplification for direct quantitation of nucleic acid sequences in clinical specimens
24. Effects of the obese gene product on body weight regulation in ob/ob mice
25. PPARgamma activation induces the expression of the adipocyte fatty acid binding protein gene in human monocytes
26. The nucleotide sequence of three genes participating in the adipose differentiation of 3T3 cells
27. A fat-specific enhancer is the primary determinant of gene expression for adipocyte P2 in vivo
28. High variability in rabbit bone marrow-derived mesenchymal cell preparations
29. PPAR-gamma: Adipogenic regulator and thiazolidinedione receptor
30. Molecular cloning of mRNA from 3T3 adipocytes. Regulation of mRNA content for glycerophosphate dehydrogenase and other differentiation-dependent proteins during adipocyte development
31. PPAR gamma 2: Tissue-specific regulator of an adipocyte enhancer
32. Mechanism of regulation of the 422(aP2) gene by cAMP during preadipocyte differentiation
33. Positional cloning of the mouse obese gene and its human homologue