Functions of SR proteins in the U12-dependent AT-AC pre-mRNA splicing pathway

RNA

Volumn 7, Issue 3, 2001, Pages 471-482

  Hastings, Michelle L ^a   Krainer, Adrian R ^a  

^a Simons Center for Quantitative Biology   (United States)

Author keywords

AT AC introns; Exon definition; Exonic splicing enhancer; Pre mRNA splicing; SR proteins

Indexed keywords

MESSENGER RNA; PROTEIN; PROTEIN SR; SODIUM CHANNEL; UNCLASSIFIED DRUG;

ARTICLE; CHIMERA; DNA FLANKING REGION; ENHANCER REGION; ENZYME ACTIVITY; EXON; INTRON; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; RNA SPLICING;

CELL-FREE SYSTEM; EXONS; HELA CELLS; HUMANS; INTRONS; NUCLEAR PROTEINS; PHOSPHOPROTEINS; RIBONUCLEOPROTEINS, SMALL NUCLEAR; RNA PRECURSORS; RNA SPLICING; RNA, MESSENGER; RNA-BINDING PROTEINS; SODIUM CHANNELS;

EID: 0035037737     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838201002552     Document Type: Article

References (45)

1. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals
2. Analysis of the RNA-recognition motif and RS and RGG domains: Conservation in metazoan pre-mRNA splicing factors
3. Exonic splicing enhancers: Mechanism of action, diversity and role in human genetic diseases
4. New proteins related to the Ser-Arg family of splicing factors
5. A coactivator of pre-mRNA splicing
6. Evolutionary fates and origins of U12-type introns
7. Initial recognition of U12-dependent introns requires both U11/5′ splice-site and U12/branchpoint interactions
8. Dynamic exchanges of RNA interactions leading to catalytic core formation in the U12-dependent spliceosome
9. Sorting out the complexity of SR protein functions
10. Evidence for substrate-specific requirement of the splicing factor U2AF(35) and for its function after polypyrimidine tract recognition by U2AF(65)
11. Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns
12. The function of multisite splicing enhancers
13. Serine-arginine (SR)-rich splicing factors have an exon-independent function in pre-mRNA splicing
14. U1 snRNP targets an essential splicing factor, U2AF65, to the 3′ splice site by a network of interactions spanning the exon
15. Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors
16. Analysis of splicing complexes and small nuclear ribonucleoprotein particles by native gel electrophoresis
17. The essential pre-mRNA splicing factor SF2 influences 5′ splice site selection by activating proximal sites
18. Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells
19. Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro
20. Exonic splicing enhancer motif recognized by human SC35 under splicing conditions
21. Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins
22. The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes
23. Purification and characterization of human RNPS1: A general activator of pre-mRNA splicing
24. Mammalian in vitro splicing assays
25. Preparation of HeLa cell nuclear and cytosolic S100 extracts for in vitro splicing
26. Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc
27. The type 2C Ser/Thr phosphatase PP2Cγ is a pre-mRNA splicing factor
28. Exon definition may facilitate splice site selection in RNAs with multiple exons
29. SR proteins escort the U4/U6.U5 tri-snRNP to the spliceosome
30. Identification and characterization of three members of the human SR family of pre-mRNA splicing factors
31. The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities
32. Modulation of 5′ splice site choice in premessenger RNA by two distinct steps
33. Highly diverged U4 and U6 small nuclear RNAs required for splicing rare AT-AC introns
34. A novel spliceosome containing U11, U12, and U5 snRNPs excises a minor class (AT-AC) intron in vitro
35. A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA
36. Identification of both shared and distinct proteins in the major and minor spliceosomes
37. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing
38. U1-mediated exon definition interactions between AT-AC and GT-AG introns
39. Splicing of a divergent subclass of AT-AC introns requires the major spliceosomal snRNAs
40. Purine-rich enhancers function in the AT-AC pre-mRNA splicing pathway and do so independently of intact U1 snRNP
41. AT-AC pre-mRNA splicing mechanisms and conservation of minor introns in voltage-gated ion channel genes
42. Functional recognition of the 3′ splice site AG by the splicing factor U2AF35
43. Purification of SR protein splicing factors
44. Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor
45. The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing