Application of a green fluorescent fusion protein to study protein-protein interactions by electrophoretic methods

Electrophoresis

Volumn 22, Issue 7, 2001, Pages 1428-1435

  Kiessig, Steffen ^a   Reissmann, Jana ^a   Rascher, Christine ^a   Küllertz, Gerhard ^a   Fischer, Andreas ^a   Thunecke, Frank ^a,b  

^a MAX PLANCK RESEARCH UNIT FOR ENZYMOLOGY OF PROTEIN FOLDING   (Germany)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Affinity capillary electrophoresis; Band shift analysis; Cyclophilin; Drosophila melanogaster; Green fluorescent protein; HIV 1 capsid protein; Laser induced fluorescence; Protein protein interaction

Indexed keywords

CAPILLARY ELECTROPHORESIS; DISSOCIATION; ENZYMES; FLUORESCENCE IMAGING; LASER OPTICS; LASER PRODUCED PLASMAS;

AFFINITY CAPILLARY ELECTROPHORESIS; BAND-SHIFT ANALYSIS; CAPSID PROTEINS; CYCLOPHILIN; DROSOPHILLA MELANOGASTER; FUSION PROTEINS; GREEN FLUORESCENT PROTEIN; HIV-1 CAPSID PROTEIN; LASER INDUCED FLUORESCENCE; PROTEIN-PROTEIN INTERACTIONS;

SIZE EXCLUSION CHROMATOGRAPHY;

CAPSID PROTEIN; CELL EXTRACT; CYCLOPHILIN; CYCLOSPORIN A; GAG PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; IMMUNOSUPPRESSIVE AGENT; INDICATOR; LIGAND;

AFFINITY CAPILLARY ELECTROPHORESIS; ARTICLE; CAPILLARY ELECTROPHORESIS; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DISSOCIATION CONSTANT; DROSOPHILA MELANOGASTER; DRUG PROTEIN BINDING; ELECTROPHORESIS; EMBRYO; ENZYME ACTIVITY; FLUORESCENCE ANALYSIS; GEL ELECTROPHORESIS; GEL MOBILITY SHIFT ASSAY; GEL PERMEATION CHROMATOGRAPHY; HUMAN IMMUNODEFICIENCY VIRUS 1; LASER; NONHUMAN; PROTEIN FAMILY; PROTEIN PROTEIN INTERACTION; SCREENING; TARGET CELL;

EID: 0035033557     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/1522-2683(200105)22:7<1428::AID-ELPS1428>3.0.CO;2-Z     Document Type: Article

References (34)