Nuclease resistance and RNase H sensitivity of oligonucleotides bridged by oligomethylenediol and oligoethylene glycol linkers

Antisense and Nucleic Acid Drug Development

Volumn 11, Issue 2, 2001, Pages 77-85

  Vorobjev, P E ^a   Pyshnaya, I A ^a   Pyshnyi, D V ^a   Venyaminova, A G ^a   Ivanova, E M ^a   Zarytova, V F ^a   Bonora, G M ^b   Scalfi Happ, C ^c   Seliger, H ^c  

^a INSTITUTE OF CHEMICAL BIOLOGY AND FUNDAMENTAL MEDICINE   (Russian Federation)

^b UNIVERSITY OF TRIESTE   (Italy)

^c UNIVERSITY OF ULM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGODEOXYNUCLEOTIDE; MACROGOL; NUCLEASE; OLIGONUCLEOTIDE; PHOSPHODIESTERASE; RIBONUCLEASE H; SNAKE VENOM;

ARTICLE; CHEMICAL MODIFICATION; ELECTROPHORESIS; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; HYDROLYSIS; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

ESCHERICHIA COLI; SERPENTES;

EID: 0035033346     PISSN: 10872906     EISSN: None     Source Type: Journal    
DOI: 10.1089/108729001750171290     Document Type: Article

References (24)

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2. Optical properties of nucleic acids, adsorption, and circular dicroism spectra
3. Comparative inhibition of rabbit globin mRNA translation by modified antisense oligodeoxynucleotides
4. α-Oligonucleotides: An entry to a challenging class of antisense molecules
5. RNA sequencing
6. Site-specific enzymatic cleavage of RNA
7. Circular dichroism studies of an oligodeoxyribonucleotide containing a hairpin loop made of a hexaethylene glycol chain: Conformation and stability
8. 12 sequences bridged by two hexaethylene glycol chains
9. α-DNA VI: Comparative study of α- andβ-anomeric oligodeoxyribonucleotides in hybridization to mRNA and in cell free translation inhibition
10. N-(2-hydroxyethyl)phenazinium derivatives of oligonucleotides as effectors of the sequence-specific modification of nucleic acids with reactive oligonucleotide derivatives
11. Antisense oligonucleotides made of 2′-O-alkylRNA: Their properties and applications in RNA biochemistry
12. RNase H-mediated inhibition of translation by antisense oligodeoxyribonucleotides: Use of back bone modification to improve specificity
13. RNase H is responsible for the non-specific inhibition of in vitro translation by 2′-O-alkyl chimeric oligonucleotides: High affinity or selectivity, a dilemma to design antisense oligomers
14. Comparative hybrid arrest by tandem antisense oligodeoxyribonucleotides or oligodeoxyribonucleosides methylphosphonates in a cell-free system
15. DNA triplex formation of oligonucleotide analogues consisting of linker groups and octamer segments that have opposite sugar-phosphate backbone polarities
16. Antisense effect of oligonucleotides with inverted terminal internucleotidic linkages: A minimal modification protecting against nucleolytic degradation
17. Oligonucleotide conjugates designed for discriminative hybridization at physiological temperature
18. Number and distribution of methylphosphonate linkages in oligodeoxynucleotides affect exo- and endonuclease sensitivity and ability to form RNase H substrates
19. Control of folding and binding of oligonucleotides by use of a nonnucleotidic linker
20. Physicochemical properties of phosphorothioate oligodeoxynucleotides
21. Artificial regulation of gene expression by complementary oligonucleotides - An overview
22. Hybridization properties of oligonucleotide pairs bridged by polyarginine peptides
23. Synthesis of oligoarginine-oligonucleotide conjugates and oligoarginine-bridged oligonucleotide pairs
24. Specificity of antisense oligonucleotides in vivo