Superfusion of synaptosomes to study presynaptic mechanisms involved in neurotransmitter release from rat brain

Brain Research Protocols

Volumn 7, Issue 2, 2001, Pages 94-102

  Garcia Sanz, A ^a   Badia, A ^a   Clos, M V ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Neuropharmacology; Neurotransmitter release; Presynaptic receptor; Superfusion; Synaptosome

Indexed keywords

4 AMINOPYRIDINE; ACETYLCHOLINE; NEUROTRANSMITTER; POTASSIUM CHANNEL; POTASSIUM ION; PRESYNAPTIC RECEPTOR; TRITIUM; VERATRIDINE;

ACETYLCHOLINE RELEASE; ANIMAL TISSUE; ARTICLE; CORPUS STRIATUM; MALE; NEUROTRANSMISSION; NEUROTRANSMITTER RELEASE; NONHUMAN; PRESYNAPTIC NERVE; PRIORITY JOURNAL; RAT; SYNAPTIC TRANSMISSION; SYNAPTOSOME;

4-AMINOPYRIDINE; ACETYLCHOLINE; ANIMALS; CHOLINE; DIFFUSION CHAMBERS, CULTURE; INFUSION PUMPS; MALE; NEUROTRANSMITTER AGENTS; POTASSIUM; PRESYNAPTIC TERMINALS; RATS; RATS, SPRAGUE-DAWLEY; SYNAPTOSOMES; TRITIUM; VERATRIDINE;

EID: 0035025651     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(00)00058-1     Document Type: Article

References (42)

1. Acquas E., Di Chiara G. Dopamine D(1) receptor-mediated control of striatal acetylcholine release by endogenous dopamine. Eur. J. Pharmacol. 383:1999;121-127.
2. 2+-independent release of neurotransmitters. J. Neurochem. 58:1992;395-405.
3. 2+-independent veratridine-evoked acetylcholine release from striatal slices is not inhibited by vesamicol (AH5183): mobilization of distinct transmitter pools. J. Neurochem. 56:1991;52-58.
4. Begley J.G., Butterfield D.A., Keller J.N., Koppal T., Drake J., Mattson M.P. Cryopreservation of rat cortical synaptosomes and analysis of glucose and glutamate transporter activities, and mitochondrial function. Brain Res. Brain Res. Protoc. 3:1998;76-82.
5. 2+ entry into PC12 cells initiated by ryanodine receptors or inositol 1,4,5-trisphosphate receptors. Biochem. J. 329:1998;349-357.
6. Bloc A., Bugnard E., Dunant Y., Falk-Vairant J. Acetylcholine synthesis and quantal release reconstituted by transfection of mediatophore and choline acetyltranferase cDNAs. Eur. J. Neurosci. 11:1999;1523-1534.
7. Bonanno G., Carita F., Cavazzani P., Munari C., Raiteri M. Selective block of rat and human neocortex GABA(B) receptors regulating somatostatin release by a GABA(B) antagonist endowed with cognition enhancing activity. Neuropharmacology. 38:1999;1789-1795.
8. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 248:1976;248-254.
9. 3H]noradrenaline release in rat hippocampal synaptosomes. Br. J. Pharmacol. 118:1996;901-904.
10. 2 dopamine receptors modulate the spontaneous acetylcholine (ACh) release from rat striatal synaptosomes. Br. J. Pharmacol. 122:1997;286-290.
11. Damsma G., Biessels P.T., Westerink B.H., De Vries J.B., Horn A.S. Differential effects of 4-aminopyridine and 2,4-diaminopyridine on the in vivo release of acetylcholine and dopamine in freely moving rats measured by intrastriatal dialysis. Eur. J. Pharmacol. 145:1988;15-20.
12. Damsma G., van Lammerts B., Westerink B.H., Horn A.S. Determination of acetylcholine and choline in the femtomole range by means of HPLC, a post-column enzyme reactor, and electrochemical detection. Chromatographia. 24:1987;827-831.
13. DiChiara G., Morelli M., Consolo S. Modulatory functions of neurotransmitters in the striatum:ACh/dopamine/NMDA interactions. Trends Neurosci. 17:1994;228-233.
14. Dolezal V., Tucek S. Calcium channels involved in the inhibition of acetylcholine release by presynaptic muscarinic receptors in rat striatum. Br. J. Pharmacol. 127:1999;1627-1632.
15. Dolezal V., Wecker L. Modulation of acetylcholine release from rat striatal slices: interaction between 4-aminopyridine and atropine. J. Pharmacol. Exp. Ther. 258:1991;762-766.
16. 2+ mobilization and release of acetylcholine guinea-pig neostriatal slices. Br. J. Pharmacol. 287:1987;297.
17. 2 dopamine receptor subtype in rat striatal synaptosomes. Brain Res. 854:2000;42-47.
18. Gleitz J., Beile A., Wilffert B., Tegtmeier F. Cryopreservation of freshly isolated synaptosomes prepared from cerebral cortex of rats. J. Neurosci. Methods. 47:1993;191-197.
19. Gray E.G., Whittaker V.P. The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation. J. Anat. 96:1962;79-88.
20. 3H]acetylcholine release by striatal glutamate and dopamine receptors. Brain Res. 844:1999;106-117.
21. Hardy J.A., Dodd P.R., Oakley A.E., Perry R.H., Edwardson J.A., Kidd A.M. Metabolically active synaptosomes can be prepared from frozen rat and human brain. J. Neurochem. 40:1983;608-614.
22. Hebb C.O., Whittaker V.P. Intracellular distributions of acetylcholine and choline acetylase. J. Physiol. 142:1958;187-196.
23. Ikarashi Y., Yuzurihara M., Takahashi A., Ishimaru H., Shiobara T., Maruyama Y. Direct regulation of acetylcholine release by N-methyl-D-aspartic acid receptors in rat striatum. Brain Res. 795:1998;215-220.
24. Israel M., Lesbats B. Application to mammalian tissues of the chemiluminescent method for detecting acetylcholine. J. Neurochem. 39:1982;248-250.
25. Kawaguchi Y., Wilson C.J., Augood S.J., Emson P.C. Striatal interneurones: chemical, physiological and morphological characterization. Trends Neurosci. 18:1995;527-535.
26. Login I.S., Borland K., Harrison M.B., Ragozzino M.E., Gold P.E. Acetylcholine release from dissociated striatal cells. Brain Res. 697:1995;271-275.
27. 1A receptors respectively mediate inhibition of glutamate release and inhibition of cyclic GMP production in rat cerebellum in vitro. J. Neurochem. 66:1996;203-209.
28. Minnema D., Michaelson I.A. A superfusion apparatus for the examination of neurotransmitter release in synaptosomes from Torpedo electric organ. J. Neurosci. Methods. 14:1985;193-206.
29. Morot-Gaudry Y., Romo R., Lesbats B., Cheramy A., Godeheu G., Glowinsky J., Israel M. Acetylcholine release in the cat caudate nucleus measured with the choline oxidase method. Eur. J. Pharmacol. 110:1985;81-87.
30. Nagy A.K., Houser C.R., Delgado-Escueta A.V. Synaptosomal ATPase activities in temporal cortex and hippocampal formation of humans with focal epilepsy. Brain Res. 529:1990;192-201.
31. Nicholls D.G. Ions and membrane potentials. Proteins, Transmitters and Synapses. 1994;26-38 Blackwell Scientific Publications, Oxford.
32. Pearce L.B., Buck T., Adamec E. Rapid kinetics of potassium-evoked release of acetylcholine from rat brain synaptosomes: analysis by rapid superfusion. J. Neurochem. 57:1991;636-647.
33. 3 histamine receptors. Naunyn Schmiedebergs Arch. Pharmacol. 360:1999;558-564.
34. Raiteri M., Angelini F., Levi G. A simple apparatus for studying the release of neurotransmitters from synaptosomes. Eur. J. Pharmacol. 25:1974;411-414.
35. Raiteri M., Marchi M., Bonanno G., Fedele E., Versace P. Dopamine release and dopaminergic inhibition of acetylcholine release in rat striatal slices after nigro-striatal hemitransection and parenteral ganglioside administration. Eur. J. Pharmacol. 213:1992;17-24.
36. Sabria J., Pastor C., Clos M.V., Garcia Sanz A., Badia A. Involvement of different types of voltage-sensitive calcium channels in the presynaptic regulation of noradrenaline release in rat brain cortex and hippocampus. J. Neurochem. 64:1995;2567-2571.
37. 2+ on acetylcholine release from rat brain synaptosomes. J. Neurochem. 56:1991;568-574.
38. Sherman A.D., Hegwood T.S., Baruah S., Waziri R. Deficient NMDA-mediated glutamate release from synaptosomes of schizophrenics. Biol. Psychiatry. 30:1991;1191-1198.
39. 2+ exchange system in basolateral membranes from rat kidney cortex. Membr. Biol. 79:1984;19-31.
40. Versteeg D.H.G., Heemskerk F.M.J., Spierenburg H.A., Graan de P.N.E., Schrama L.H. 4-Aminopyridine differentially affects the spontaneous release of radiolabelled transmitters from rat brain slices in vitro. Brain Res. 686:1995;233-238.
41. Whittaker V.P. Thirty years of synaptosome research. J. Neurocytol. 22:1993;735-742.
42. Yamada S., Yokoto H., Nishi S. Differential effects of dopamine agonists on evoked dopamine release from slices of striatum and nucleus accumbens in rats. Brain Res. 648:1994;176-179.