Purification and biochemical characterization of a fibrinolytic enzyme from Bacillus subtilis BK-17

World Journal of Microbiology and Biotechnology

Volumn 17, Issue 1, 2001, Pages 89-92

  Jeong, Yong Kee ^a   Park, Jeong Uck ^b   Baek, Hyun ^a   Park, Sung Hoon ^c   Kong, In Soo ^d   Kim, Dong Wan ^e   Joo, Woo Hong ^e  

^a Dong Eui University   (South Korea)

^b Gyeongsang National University School of Medicine   (South Korea)

^c Pusan National University   (South Korea)

^d Pukyong National University   (South Korea)

^e Changwon National University   (South Korea)

Author keywords

Amidolytic activity; Bacillus subtilis BK 17; Fibrinolytic enzyme; Homologous proteins; N terminal amino acid sequencing; Protease inhibitor; Thrombolytic agent

Indexed keywords

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; BACTERIAL ENZYME; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SUBSTRATE; ENZYME SUBUNIT; GEL FILTRATION CHROMATOGRAPHY; GENETIC STRAIN; ION EXCHANGE CHROMATOGRAPHY; KALLIKREIN; MOLECULAR WEIGHT; NATTOKINASE; PLASMIN; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE HOMOLOGY; SUBTILISIN;

EID: 0035024732     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1016685411809     Document Type: Article

References (20)

1. Basic local alignment search tool
2. Effect of staphylokinase concentration of plasminogen activation
3. The fibrin plate method for estimating fibrinolytic activity
4. The crystal structure of an autoprocessed Ser221Cys-subtilisin. E-propeptide complex at 2.0-A resolution
5. Molecular cloning of a subtilisin J gene from Bacillus stearothermophilus and its expression in Bacillus subtilis
6. Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang
7. Purification and characterization of a novel fibrinolytic enzyme from Bacillus sp. KA38 originated from fermented fish
8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
9. On the mechanism of fibrin-specific plasminogen activation by staphylokinase
10. Domain structure, stability and interactions in streptokinase
11. Nucleotide sequence of the subtilisin NAT gene, aprN of Bacillus subtilis (natto)
12. Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli
13. 125I-labeled human high molecular weight urokinase across the intestinal tract in a dog model with stimulation of synthesis and/or release of plasminogen activators
14. Activation of plasma fibrinolysis after intrarectal administration of high molecular weight urokinase and its derivative
15. Transport of urokinase across the intestinal tract of normal human subjects with stimulation of synthesis and/or release of urokinase-type proteins
16. Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein
17. The subtilisin E gene of Bacillus subtilis is transcribed from a sigma-37 promoter in vivo
18. Isolation and characterization of urokinase from human plasma
19. A mutant subtilisin E with enhanced thermostability
20. Cloning and expression of subtilisin amylosacchariticus gene