Fluorescent staining of glycoproteins on polyvinylidene difluoride membrane with 8-aminonaphthalene-1,3,6-trisulfonate

BioTechniques

Volumn 30, Issue 6, 2001, Pages 1272-1278

  Che, F Y ^a   Song, J F ^a   Yu, L R ^a   Wang, K Y ^a   Xia, Q C ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMINATION; CARBOHYDRATES; FLUORESCENCE; POLYMERIC MEMBRANES; PROTEINS; SENSITIVITY ANALYSIS;

PERIODIC ACID OXIDATION;

BIOTECHNOLOGY;

8 AMINO 1,3,6 NAPHTHALENETRISULFONIC ACID; CARBOHYDRATE; FLUORESCENT DYE; GLYCOPROTEIN; LECTIN; NAPHTHALENE DERIVATIVE; PERIODIC ACID; POLYVINYLIDENE FLUORIDE; UNCLASSIFIED DRUG; WHEAT GERM AGGLUTININ;

AMINATION; ANALYTIC METHOD; ARTICLE; ARTIFICIAL MEMBRANE; OXIDATION; REACTION ANALYSIS; STAINING;

FORMICIDAE; TRITICUM AESTIVUM;

EID: 0035016236     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01306st05     Document Type: Article

References (28)

1. Enzyme-based detection of glycoproteins on blot transfers using avidin-biotin technology
2. Blot analysis of glycoconjugates using digoxigenin-labeled lectins: An optimized procedure
3. The interaction of wheat germ agglutinin with sialogycoproteins. The role of sialic acid
4. Cell surface carbohydrates in cell recognition and response
5. Hydrazinoacridine staining of proteins and glycoproteins in polyacrylamide gels
6. Quantitative analysis of sugar constituents of glycoproteins by capillary electrophoresis
7. Glycosylation of recombinant protein therapeutics: Control and functional implications
8. A sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gels
9. Staining of glycoproteins in polyacrylamide and agarose gels with fluorescent lectins
10. Blot analyses of glycoconjugates: Enzyme-hydrazide - A novel reagent for the detection of aldehydes
11. A lectin-binding assay for the rapid characterization of the glycosylation of purified glycoproteins
12. Identification of glycoproteins on nitrocellulose membranes using lectin blotting
13. Analysis of glycoproteins separated by two-dimensional gel electrophoresis using lectin blotting revealed by chemiluminescence
14. Structural characterization of glycoprotein carbohydrate chains by using digoxigenin-labeled lectins on blots
15. Fluorophore-assisted carbohydrate electrophoresis technology and applications
16. The use of polyacrylamide-gel electrophoresis for the high-resolution separation of reducing saccharides labeled with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid: Detection of picomolar quantities by an imaging system based on a cooled charge-coupled device
17. An ultrasensitive technique for the analysis of glycoproteins using lecting blotting enchanced chemiluminescence
18. Simple method for detecting glycoproteins dot-blotted or electro-blotted on to a polyvinylidene difluoride membrane
19. Separation of 8-aminonaphthalene-1,3,6-trisulfonic acid-labeled neutral and sialylated N-linked complex oligosaccharides by capillary electrophoresis
20. Selective staining of human platelet glycoproteins using nitrocellulose transfer of electrophoresed proteins and peroxidase-conjugated lectins
21. Quantitation of glycoproteins on electroblots using the biotin-streptavidin complex
22. Therapeutic challenges: Does glycobiology have a role?
23. Effect of glycosylation on protein function
24. Periodic acid/Schiff staining of glycoproteins immobilized on a blotting matrix
25. Two-dimensional mapping of N-glycosidically linked asialo-oligosaccharides from glycoproteins as reductively pyridylaminated derivatives using dual separation modes of high-performance capillary electrophoresis
26. Biological roles of oligosaccharides: All of the theories are correct
27. The staining of sciatic nerve glycoproteins on polyacrylamide gels with concanavalin A-peroxidase
28. Glycoprotein staining following electrophoresis on acrylamide gel