Activation of arrestin: Requirement of phosphorylation as the negative charge on residues in synthetic peptides from the carboxyl-terminal region of rhodopsin

Investigative Ophthalmology and Visual Science

Volumn 42, Issue 7, 2001, Pages 1439-1443

  McDowell, J H ^a   Robinson, P R ^a   Miller, R L ^a   Brannock, M T ^a   Arendt, A ^a   Smith, W C ^a   Hargrave, P A ^a  

^a UNIVERSITY OF FLORIDA HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; ASPARTIC ACID; CYSTEIC ACID; GLUTAMIC ACID; PHOSPHODIESTERASE; PHOSPHOSERINE; PHOSPHOTHREONINE; RETINA S ANTIGEN; RHODOPSIN; SYNTHETIC PEPTIDE; THIOL GROUP; TRANSDUCIN;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; RETINA ROD OUTER SEGMENT;

3',5'-CYCLIC-GMP PHOSPHODIESTERASE; ANIMALS; ARRESTIN; ASPARTIC ACID; CATTLE; CYCLIC GMP; CYSTEIC ACID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLUTAMIC ACID; PEPTIDE FRAGMENTS; PHOSPHORYLATION; PHOTOTRANSDUCTION; RHODOPSIN; ROD OUTER SEGMENTS; SULFHYDRYL COMPOUNDS;

EID: 0035013151     PISSN: 01460404     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Structure and functions of arrestins
2. Synthetic phosphopeptide from rhodopsin sequence induces retinal arrestin binding to photoactivated unphosphorylated rhodopsin
3. Sulfhydryl reactivity demonstrates different conformational states for arrestin, arrestin activated by a synthetic phosphopeptide, and constitutively active arrestin
4. Phosphorylated rhodopsin and heparin induce similar conformational changes in arrestin
5. Synthesis of multiphosphorylated peptide from a C-terminal bovine rhodopsin sequence
6. Substitution of cysteic acid for the seven phosphorylated residues in bovine rhodopsin's C-terminal peptide
7. Synthesis of peptide with multiple cysteic acids
8. The isolation and purification of osmotically intact discs from retinal rod outer segments
9. Purification of arrestin from bovine retinas
10. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
11. Rhodopsin's carboxyl-terminal threonines are required for wild-type arrestin-mediated quench of transducin activation in vitro
12. Rhodopsin phosphorylation and dephosphorylation in vivo
13. Rapid and reproducible deactivation of rhodopsin requires multiple phosphorylation sites
14. Rhodopsin phosphorylation sites and their role in arrestin binding