Development of single-chain Fv fragments from a human anti-double-stranded DNA antibody to study the influence of somatic mutations on antigen binding

Experimental and Clinical Immunogenetics

Volumn 18, Issue 2, 2001, Pages 96-99

  Kersten, Birgit ^a,b   Niemann, B ^a,c   Jahn, S ^a,d  

^a HUMBOLDT UNIVERSITY   (Germany)

^b MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

^c Fed Inst Hlth Protect C   (Germany)

^d Asche AG   (Germany)

Author keywords

Affinity; Anti dsDNA antibody; Expression; Human autoantibody; scFv fragments; Somatic mutation

Indexed keywords

AUTOANTIBODY; DNA ANTIBODY; DOUBLE STRANDED DNA ANTIBODY; IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN FV FRAGMENT; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY 32B9; UNCLASSIFIED DRUG;

ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; ANTIGEN BINDING; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENE EXPRESSION; GENE MUTATION; GENETIC TRANSFECTION; PRIORITY JOURNAL; SOMATIC MUTATION; STRUCTURE ACTIVITY RELATION; SURFACE PLASMON RESONANCE; SYSTEMIC LUPUS ERYTHEMATOSUS;

ANTIBODIES, ANTINUCLEAR; ANTIGEN-ANTIBODY REACTIONS; BINDING SITES, ANTIBODY; DNA; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; LUPUS ERYTHEMATOSUS, SYSTEMIC; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT FUSION PROTEINS;

EID: 0035012828     PISSN: 02549670     EISSN: None     Source Type: Journal    
DOI: 10.1159/000049188     Document Type: Article

References (8)

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