Terminal sialylation is altered in airway cells with impaired CFTR-mediated chloride transport

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 280, Issue 3 24-3, 2001, Pages

  Kube, Dianne ^a,b   Adams, Lynn ^a   Perez, Aura ^a   Davis, Pamela B ^a  

^a CASE WESTERN RESERVE UNIVERSITY   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

3 (2,4 dinitroanilino) 3 amino N methyldipropylamine; Cystic fibrosis; Cystic fibrosis transmembrane conductance regulator; Fluorescent imaging; Immunogold labeling; Lectins; Regulatory domain; F508

Indexed keywords

SIALIC ACID; TRANSMEMBRANE CONDUCTANCE REGULATOR;

AIRWAY; ARTICLE; CHLORIDE TRANSPORT; CONTROLLED STUDY; EPITHELIUM CELL; GENE OVEREXPRESSION; GENETIC TRANSFECTION; GLYCOSYLATION; HUMAN; HUMAN CELL; LECTIN BINDING; PRIORITY JOURNAL; PROTEIN DOMAIN; SIALYLATION; TRACHEA MUCOSA;

EID: 0035006957     PISSN: 10400605     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajplung.2001.280.3.l482     Document Type: Article

References (32)

1. Aatsinki JR, Lakkakorpi JT, Pietila EM, and Rajaniemi HJ. A coupled one-step reverse transcription PCR procedure for generation of full-length open reading frames. Biotechniques 16: 283-288, 1994.
2. Al-Awqati Q, Barasch J, and Landry D. Chloride channels of intracellular organelles and their potential role in cystic fibrosis. J Exp Biol 172: 245-266, 1992.
3. Anderson RGW and Pathak RK. Vesicles and cisternae in the trans-Golgi apparatus of human fibroblasts are acidic compartments. Cell 40: 635-643, 1985.
4. Barasch J and Al-Awqati Q. Defective acidification of the biosynthetic pathway in cystic fibrosis. J Cell Sci Suppl 17: 229-233, 1993.
5. Barasch J, Kiss B, Prince A, Saiman L, Gruenert D, and Al-Awqati Q. Defective acidification of intracellular organelles in cystic fibrosis. Nature 352: 70-73, 1991.
6. Bhavanandan VP and Katlic AW. The interaction of wheat germ agglutinin with sialoglycoproteins. The role of sialic acid. J Biol Chem 254: 4000-4008, 1979.
7. - transport in cystic fibrosis respiratory epithelia. J Clin Invest 78: 1245-1252, 1986.
8. Bryan R, Kube D, Perez A, Davis P, and Prince A. Overproduction of the CFTR R domain leads to increased levels of asioloGM1 and increased Pseudomonas aeruginosa binding by epithelial cells. Am J Respir Cell Mol Biol 19: 269-277, 1998.
9. Dosanjh A, Lencer W, Brown D, Ausiello DA, and Stow JL. Heterologous expression of ΔF508 CFTR results in decreased sialylation of membrane glycoconjugates. Am J Physiol Cell Physiol 266: C360-C366, 1994.
10. Eskandari S, Wright EM, Kremar M, Starace DM, and Zampighi GA. Structural analysis of cloned plasma membrane proteins by freeze-fracture electron microscopy. Proc Natl Acad Sci USA 95: 11235-11240, 1998.
11. Gabriel SE, Clark IL, Boucher RC, and Stutts MJ. CFTR and outward rectifying chloride channels are distinct proteins with a regulatory relationship. Nature 363: 263-266, 1993.
12. Goldstein IJ and Hayes CE. The lectins: carbohydrate-binding proteins of plants and animals. Adv Carbohydr Chem Biochem 35: 127-340, 1978.
13. i Regulation Differs in Normal and CF Phenotype Pancreatic Ductal Cells (MSc thesis). Cleveland, OH: Case Western Reserve University, 1995.
14. Knibbs RN, Goldstein IJ, Ratcliffe RM, and Shibuya N. Characterization of the carbohydrate binding specificity of the leukoagglutinating lectin from Maackia amurensis: comparison with other sialic acid-specific lectins. J Biol Chem 266: 83-88, 1991.
15. Ma JJ, Tasch JE, Tao T, Zhao JY, Xie JX, Drumm ML, and Davis PB. Phosphorylation-dependent block of cystic fibrosis transmembrane conductance regulator chloride channel by exogenous R domain protein. J Biol Chem 271: 7351-7356, 1996.
16. Ma J, Zhao J, Drumm ML, Xie J, and Davis PB. Function of the R domain in the CFTR chloride channel. J Biol Chem 272: 28133-28141, 1997.
17. - cells in culture. Am J Physiol Lung Cell Mol Physiol 271: L85-L92, 1996.
18. Pilewski JM and Frizzell RA. How do cystic fibrosis transmembrane conductance regulator mutations produce lung disease? Curr Opin Pulm Med 1: 435-443, 1995.
19. Puchelle E, de Bentzmann S, and Zahm JM. Physical and functional properties of airway secretions in cystic fibrosis -therapeutic approaches. Respiration 62: 2-12, 1995.
20. Saiman L, Cacalano G, Gruenert D, and Prince AL. Comparison of adherence of Pseudomonas aeruginosa to respiratory epithelial cells from cystic fibrosis patients and healthy subjects. Infect Immun 60: 2808-2814, 1992.
21. Saiman L and Prince A. Pseudomonas aeruginosa pili bind to asialoGM1 which is increased on the surface of cystic fibrosis epithelial cells. J Clin Invest 92: 1875-1880, 1996.
22. Sata T, Lackie PM, Taatjes DJ, Peumans WJ, and Roth J. Detection of the NeuAc (α2,3) Gal (β1,4) GlcNAc sequence with the leukoagglutinin from Maackia amurensis: light and electron microscopic demonstration of differential tissue expression of terminal sialic acid in α2,3 and α2,6 linkage. J Histochem Cytochem 37: 1577-1588, 1989.
23. Schengrund CL and Ringler NJ. Binding of Vibrio cholera toxin and the heat-labile enterotoxin of Escherichia coli to GM1, derivatives of GM1, and nonlipid oligosaccharide polyvalent ligands. J Biol Chem 264: 13233-13237, 1989.
24. Schwiebert EM, Egan ME, Hwang TH, Fulmer SB, Allen SS, Cutting GR, and Guggino WB. CFTR regulates outwardly rectifying chloride channels through an autocrine mechanism involving ATP. Cell 81: 1063-1073, 1995.
25. Seksek O, Biwersi J, and Verkman AS. Evidence against defective trans-Golgi acidification in cystic fibrosis. J Biol Chem 271: 15542-15548, 1996.
26. Shibuya J, Goldstein IJ, Broekaert WF, Nsimba-Lubaki M, Peeters B, and Peumans WJ. The elderberry (Sambucus nigra L.) bark lectin recognizes the Neu5Ac (α2,6) Gal/GalNAc sequence. J Biol Chem 262: 1596-1601, 1987.
27. Teneberg S, Angstrom J, Jovall PA, and Karlsson KA. Characterization of binding of Gal β 4GlcNAc-specific lectins from Erythrina cristagalli and Erythrina corallodendron to glycosphingolipids. Detection, isolation, and characterization of a novel glycosphingolipid of bovine buttermilk. J Biol Chem 269: 8554-8563, 1994.
28. + conductances in salt-secreting epithelial cells. Am J Physiol Cell Physiol 259: C358-C364, 1990.
29. Weyer P, Barasch J, Al-Awqati Q, Ausiello DA, and Brown D. Immunolocalization of two sialyltransferases is altered in polarized LLC-PK1 epithelial cells expressing ΔF508 CFTR (Abstract). Pediatr Pulmonol Suppl 12: 238, 1995.
30. Young IT. Proof without prejudice: use of the Kolmogorov-Smirnov test for analysis of histograms from flow systems and other sources. J Histochem Cytochem 25: 935-941, 1977.
31. Zar H, Saiman L, Quittell L, and Prince A. Binding of Pseudomonas aeruginosa to respiratory epithelial cells from patients with various mutations in the cystic fibrosis transmembrane regulator. J Pediatr 126: 230-233, 1995.
32. Zerhusen G, Zhao J, Xie J, Davis PB, and Ma J. A single conductance pore for chloride ion formed by two CFTR molecules. J Biol Chem 274: 7627-7630, 1999.