End-labeling of long DNA fragments with biotin and detection of DNA immobilized on magnetic beads

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 17, Issue 2, 2001, Pages 183-185

  Xu, Haiming ^b   Zhang, Shen ^b   Liu, Depei ^a   Liang, Chih Chuan ^b  

^a INSTITUTE OF BASIC MEDICAL SCIENCES   (China)

^b NONE

Author keywords

Biotin; DNA labeling; Globin gene; Ligation; Magnetic beads

Indexed keywords

BIOTIN; DNA; DNA FRAGMENT; STREPTAVIDIN;

DNA DETERMINATION; DNA PROTEIN COMPLEX; GLOBIN GENE; MOLECULAR INTERACTION; POLYMERASE CHAIN REACTION; QUANTITATIVE ASSAY; REVIEW;

BASE SEQUENCE; BIOTECHNOLOGY; BIOTIN; DNA; DNA PRIMERS; GLOBINS; HUMANS; MAGNETICS; MICROSPHERES; POLYMERASE CHAIN REACTION; STREPTAVIDIN;

EID: 0035004773     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:17:2:183     Document Type: Review

References (8)

1. 1. Schussler, P., Gohr, L. G., sommer, G., Kunz, W., and Grevelding, C. G. (1995) Combined isolation of nucleic acids and protein from small amounts of tissue. Trends Genet. 11, 378-379.
2. 2. Rodriguez, I. R. and Chader, G. J. (1992) A novel method for the isolation of tissue-specific genes. Nucleic Acids Res. 20, 3528.
3. 3. Gershon, P. D. and Moss, B. (1990) Early transcription factor subunits are encoded by vaccinia virus late gene. Proc. Natl. Acad. Sci. USA 87, 4401-4405.
4. 4. Sandaltzopoulos, R. and Becker, P. B. (1994) Solid phase DNase I footprinting: quick and versatile. Nucleic Acids Res. 22, 1511, 1512.
5. 5. Sandaltzopoulos, R., Blank, T., and Becker, P. B. (1994) Transcriptional repression by nucleosomes but not H1 in reconstituted preplastoderm Drosophila chromatin. EMBO J. 13, 373-379.
6. 6. Gabrielsen, O. S. and Huet, J. (1993) Magnetic DNA affinity purification of yeast transcription factor. Methods Enzymol. 218, 508-525.
7. 7. Ren, L., Chen, H., and Sternberg, E. A. (1994) Tethered bandshift assay and affinity purification of a new DNA-binding protein. BioTechniques 16, 852-855.
8. 8. Talbot, D., Collis, P., Antoniou, M., Vidal, M., Grosveld, F., and Greaves, D. R. (1989) A dominant control region from the human beta-globin locus conferring integration site-independent gene expression. Nature 338, 352-355.