Specific amplification of mRNA splice variants by RT-PCR

BioTechniques

Volumn 30, Issue 5, 2001, Pages 944-948

  Goel, Ashish ^a   Seth, Puneet ^a   Chauhan, Shyam S ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA;

ALTERNATIVE RNA SPLICING; ARTICLE; GENE AMPLIFICATION; HUMAN; KINETICS; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA SPLICING; RNA STABILITY;

EID: 0035002948     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01305bm04     Document Type: Article

References (4)

1. Chauhan, S.S., N.C. Popescu, D. Ray, R. Fleischmann, M.M. Gottesman, and B.R. Troen. 1993. Cloning, genomic organization, and chromosomal localization of human cathepsin L. J. Biol. Chem. 268:1039-1045.
2. Chomczynski, P. and N. Sacchi. 1987. Single step method of RNA isolation by acid guanidium thiocynate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
3. 145, a secreted vascular endothelial growth factor isoform that binds to extracellular matrix. J. Biol. Chem. 272:7151-7158.
4. Rescheleit, D.K., W.J. Rommerskirch, and B. Wiederanders. 1996. Sequence analysis and distribution of two new human cathepsin L splice variants. FEBS Lett. 394:345-348.