Inducible model to study negative strand RNA synthesis and assembly of hepatitis C virus from a full-length cDNA clone

Journal of Virological Methods

Volumn 94, Issue 1-2, 2001, Pages 55-67

  Myung, Jane ^a   Khalap, Nutan ^a   Kalkeri, Gururaj ^a   Garry, Robert ^a   Dash, Srikanta ^a  

^a TULANE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

Author keywords

Adex CAT7; Hepatitis C virus; Immunoelectron microscopy; Immunofluorescence; Negative strand RNA; Virus like particles; Western blot

Indexed keywords

RIBOZYME; VIRUS ANTIBODY; VIRUS RNA;

ADENOVIRUS; ARTICLE; CELL STRAIN HEPG2; CONTROLLED STUDY; DNA TRANSFECTION; HEPATITIS C VIRUS; HEPATITIS DELTA VIRUS; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE TEST; IN VITRO STUDY; MOLECULAR CLONING; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RIBONUCLEASE PROTECTION ASSAY; RNA SYNTHESIS; RNA TRANSCRIPTION; STRUCTURAL GENE; VIRUS ASSEMBLY; VIRUS DETECTION; VIRUS GENOME; VIRUS PARTICLE; VIRUS REPLICATION; WESTERN BLOTTING;

CLONING, MOLECULAR; DNA, VIRAL; HEPACIVIRUS; HUMANS; RNA, VIRAL; TRANSFECTION; TUMOR CELLS, CULTURED; VIRAL ENVELOPE PROTEINS; VIRAL NONSTRUCTURAL PROTEINS; VIRION; VIRUS ASSEMBLY;

EID: 0035002162     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(01)00278-6     Document Type: Article

References (33)

1. A human liver cell line exhibits efficient translation of HCV RNAs produced by a recombinant adenovirus expressing T7 RNA polymerase
2. Rescue of a segmented negative-strand RNA virus entirely from cloned complementary DNAs
3. Sequence analysis of the 5′ noncoding region of hepatitis C virus
4. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome
5. Genetic organization and diversity of the hepatitis C virus
6. Molecular virology of hepatitis C virus
7. In vitro infection of peripheral blood mononuclear cells by hepatitis C virus
8. Transfection of HepG2 cells with infectious hepatitis C virus genome
9. Complete 5′ noncoding region is necessary for the efficient internal initiation of hepatitis C virus RNA
10. Expression and identification of hepatitis C virus polyprotein cleavage products
11. Characterization of the terminal regions of hepatitis c viral RNA: Identification of conserved sequences in the 5′ untranslated regions and poly(A) tails at the 3′ end
12. Characterization of an established human hepatoma cell line constitutively expressing non-structural proteins of hepatitis C virus by transfection of viral cDNA
13. Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis
14. HCV cDNA transfection to HepG2 cells
15. Structural requirements for initiation of translation by internal ribosome entry within genome-length hepatitis c virus RNA
16. Hepatitis C viruses
17. Cultivation of hepatitis C virus in primary hepatocyte culture from patients with chronic hepatitis C results in release of high tire infectious virus
18. Hepatitis C virus particle detected by immunoelectron microscopic study
19. Identification of a highly conserved sequence element at the 3′ terminus of hepatitis C virus genome RNA
20. Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT/PCR
21. Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line
22. Virionlike structures in Hela G cells transfected with the full-length sequence of the hepatitis C virus genome
23. Long-term human T cell culture system supporting hepatitis C virus replication
24. Tightly regulated expression of the entire hepatitis C virus structural region in continuous human cell lines
25. Detection and quantitation of human respiratory syncytial virus (RSV) using minigenome cDNA and a Sindbis virus replicon: A prototype assay for negative-strand RNA viruses
26. Almost the entire 5′ nontranslated region of hepatitis C virus is required for cap independent translation
27. Hepatitis C virus infection is associated with the development of hepatocellular carcinoma
28. Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line
29. Hepatitis C virus: Detection of intracellular virus particles by electron microscopy
30. Structure of the 3′ terminus of the hepatitis C virus genome
31. Expression of target genes by coinfection with replication-deficient viral vectors
32. Transfection of a differentiated human hepatoma cell line (Huh-7) with in vitrotranscribed hepatitis C virus (HCV) RNA and establishment of a long-term culture persistently infected with HCV