Folding screening assayed by proteolysis: Application to various cystine deletion mutants of vascular endothelial growth factor

Protein Engineering

Volumn 14, Issue 3, 2001, Pages 183-188

  Heiring, Christoph ^a   Muller, Yves A ^a  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

Cystine knot growth factor family; Inclusion bodies; Proteolysis; Renaturing; Screening

Indexed keywords

CYSTINE; RECOMBINANT PROTEIN; VASCULOTROPIN;

ARTICLE; DELETION MUTANT; ESCHERICHIA COLI; HYDROPHOBICITY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FAMILY; PROTEIN FOLDING; PROTEIN RENATURATION; PROTEIN STABILITY; TECHNIQUE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0034999062     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/14.3.183     Document Type: Article

References (17)

1. Armstrong, N., de Lencastre, A. and Gouaux, E. (1999) Protein Sci., 8, 1475-1483.
2. Caspers, P., Stieger, M. and Burn, P. (1994) Cell. Mol. Biol., 40, 635-644.
3. Chen, G.Q. and Gouaux, E. (1997) Proc. Natl Acad. Sci. USA, 94, 13431-13436.
4. Christinger, H.W., Muller, Y.A., Berleau, L., Keyt, B.A., Cunningham, B.C., Ferrara, N. and de Vos, A.M. (1996) Proteins, 26, 353-357.
5. Kraulis, P.J. (1991) J. Appl. Crystallogr., 24, 946-950.
6. Lilie, H., Schwarz, E. and Rudolph, R. (1998) Curr. Opin. Biotechnol., 9, 497-501.
7. McDonald, N.Q. and Hendrickson, W.A. (1993) Cell, 73, 421-424.
8. Merritt, E.A. and Murphy, M.E.P. (1994) Acta Crystallogr., D50, 869-873.
9. Muller, Y.A., Christinger, H.W., Keyt, B.A. and de Vos, A.M. (1997) Structure, 5, 1325-1338.
10. Nakagawa, N., Masui, R., Kato, R. and Kuramitsu, S. (1997) J. Biol. Chem., 272, 22703-22713.
11. Ooi, T., Rupley, J.A. and Scheraga, H.A. (1963) Biochemistry, 2, 432-437.
12. Porter, R.R. (1973) Science, 180, 713-716.
13. Rudolph, R. and Lilie, H. (1996) FASEB J., 10, 49-56.
14. Rudolph, R., Böhm, G., Lilie, H. and Jaenicke, R. (1997) In Creighton, T.E. (ed.),Protein Function, a Practical Approach. 2nd edn. IRL Press, Oxford, pp. 57-99.
15. Schmid, F. and Blaschek, H. (1984) Biochemistry, 23, 2128-2133.
16. Schwartz, T., Lowenhaupt, K., Kim, Y.G., Li, L., Brown, B.A.,II, Herbert, A. and Rich, A. (1999) J. Biol. Chem., 274, 2899-2906.
17. Teschner, W. and Rudolph, R. (1989) Biochem. J., 260, 583-587.