Mutational analysis of the high-affinity BvgA binding site in the fha promoter of Bordetella pertussis

Molecular Microbiology

Volumn 40, Issue 4, 2001, Pages 991-999

  Boucher, P E ^a   Yang, M S ^a   Stibitz, S ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; AMINO ACID; CYTOSINE; MAGNESIUM SULFATE; THYMIDINE;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIUM CULTURE; BINDING AFFINITY; BORDETELLA PERTUSSIS; GENE ACTIVATION; MOLECULAR RECOGNITION; NONHUMAN; OPERON; POINT MUTATION; PRIORITY JOURNAL; PROMOTER REGION;

BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; BORDETELLA PERTUSSIS; DNA MUTATIONAL ANALYSIS; FIMBRIAE PROTEINS; MAGNESIUM SULFATE; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES; OPERON; PROMOTER REGIONS (GENETICS); TRANSCRIPTION FACTORS;

BACTERIA (MICROORGANISMS); BORDETELLA PERTUSSIS; NEGIBACTERIA;

EID: 0034995688     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2001.02442.x     Document Type: Article

References (18)

1. In vivo characterization of the unorthodox BvgS two-component sensor protein of Bordetella pertussis
2. Synergistic binding of RNA polymerase and BvgA-phosphate to the pertussis toxin promoter of Bordetella pertussis
3. The modular architecture of bacterial response regulators
4. Nature of DNA binding and RNA polymerase interaction of the Bordetella pertussis BvgA transcriptional activator at the fha promoter
5. Genetic and biochemical analysis of BvgA: Interaction with the secondary binding region of the fha promoter of Bordetella pertussis
6. Defining a bacteriophage T4 late promoter: Absence of a -35′ region
7. Antigenic modulation of Bordetella pertussis
8. Identification of Bordetella pertussis regulatory sequences required for transcriptional activation of the bvgAS operon
9. Positive transcriptional feedback at the bvg locus controls the expression of virulence factors in Bordetella pertussis
10. Sequential activation and environmental regulation of virulence genes in Bordetella pertussis
11. Phosphorylated BvgA is sufficient for transcriptional activation of virulence-regulated genes in Bordetella pertussis
12. Enzymatic inverse PCR: A restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis
13. Mutations affecting the α subunit of Bordetella pertussis RNA polymerase suppress growth inhibition conferred by short C-terminal deletions of the response regulator BvgA
14. Hfr mapping of mutations in Bordetella pertussis that define a genetic locus involved in virulence gene regulation
15. Coordinate regulation of virulence in Bordetella pertussis mediated by the vir (bvg) locus
16. Subcellular localization and immunological detection of proteins encoded by the vir locus of Bordetella pertussis
17. Differential binding of BvgA to two classes of virulence genes of Bordetella pertussis directs promoter selectivity by RNA polymerase