13C incorporation into DNA as a means of identifying the active components of ammonia-oxidizer populations

Letters in Applied Microbiology

Volumn 32, Issue 6, 2001, Pages 398-401

  Whitby, C B ^a,b,c   Hall, G ^a,b,c   Pickup, R ^a,b,c   Saunders, J R ^a,b,c   Ineson, P ^a,b,c   Parekh, N R ^a,b,c   McCarthy, A ^a,b,c  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

^b CENTRE FOR ECOLOGY AND HYDROLOGY   (United Kingdom)

^c UNIVERSITY OF YORK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; CARBON DIOXIDE; CESIUM COMPOUNDS; CHLORINE COMPOUNDS; ELECTROPHORESIS;

ACTIVE COMPONENTS; AMMONIA OXIDIZERS; AMMONIA OXIDIZING BACTERIA; C INCORPORATION; DENSITY GRADIENTS; DNA EXTRACTS; ENRICHMENT CULTURE; FRESH WATER; ULTRACENTRIFUGATION; WATER-SEDIMENTS;

DNA;

AMMONIA; CARBON 13; CARBON DIOXIDE; CESIUM CHLORIDE; DNA; RNA;

AMMONIA FORMATION; ARTICLE; DNA DETERMINATION; GEL ELECTROPHORESIS; GENE AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; SEQUENCE ANALYSIS; SPECIES DIFFERENCE; TAXONOMY;

NITROSOMONADALES;

EID: 0034995535     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.2001.00930.x     Document Type: Article

References (18)

1. Automated analysis of light-element stable isotopes by isotope ratio mass spectrometry
2. Diversity in the ammonia-oxidising nitrifier population of a soil
3. Amplification of DNA from native populations of soil bacteria by using the polymerase chain reaction
4. Application of molecular biological techniques to a seasonal study of ammonia oxidation in a eutrophic freshwater lake
5. The phylogeny of autotrophic ammonia-oxidising bacteria as determined by analysis of 16S ribosomal RNA gene sequences
6. Amplification of 16S ribosomal RNA genes of the autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment
7. Identification of major sub-groups of ammonia-oxidising bacteria in environmental samples by T-RFLP analysis of amoA PCR products
8. Analysis of ammonia-oxidising bacteria of the β-subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments
9. Sequence of the gene encoding for ammonia monooxygenase in Nitrosomonas europaea
10. Phylogeny of all recognised species of ammonia oxidiser based on comparative 16S rRNA and amoA sequence analysis: Implications for molecular surveys
11. Stable-isotope probing as a tool in microbial ecology
12. The ammonia monooxygenase structural gene amoA as a functional marker: Molecular fine scale analysis of the natural ammonia-oxidising populations
13. Molecular diversity of soil and marine 16S rRNA gene sequences related to β-subgroup ammonia-oxidising bacteria
14. In situ identification of ammonia-oxidising bacteria
15. Comparisons of the morphology and deoxyribonucleic acid components of 27 strains of nitrifying bacteria
16. Phylogenetic differentiation of two closely related Nitrosomonas spp. that inhabit different sediment environments in an oligotrophic freshwater lake