High levels of transgene expression following transduction of long-term NOD/SCID-repopulating human cells with a modified lentiviral vector

Stem Cells

Volumn 19, Issue 3, 2001, Pages 247-259

  Gao, Zhigang ^a   Golob, Jonathan ^a   Tanavde, Vivek M ^a   Civin, Curt I ^a   Hawley, Robert G ^b   Cheng, Linzhao ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b HOLLAND LABORATORY   (United States)

Author keywords

Gene therapy; Hematopoietic stem cells; Lentiviral vectors; NOD SCID mice; Progenitor cells; Transplantation

Indexed keywords

CD34 ANTIGEN; GREEN FLUORESCENT PROTEIN; VIRUS VECTOR;

ANIMAL MODEL; ARTICLE; FLOW CYTOMETRY; GENE EXPRESSION; GENE THERAPY; GENE TRANSFER; GENETIC TRANSDUCTION; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; LENTIVIRINAE; MOUSE; NONHUMAN; SCID MOUSE; VIRUS REPLICATION;

EID: 0034994357     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1634/stemcells.19-3-247     Document Type: Article

References (42)

1. + cells expands progenitor cell numbers, preserves engraftment capacity in NOD/SCID mice, and enhances retroviral transduction efficiency
2. Versatile retroviral vectors for potential use in gene therapy
3. Sustained gene expression in retrovirally-transduced, engrafting human hematopoietic stem cells and their lympho-myeloid progeny
4. Expansion of human cord blood CD34(+)CD38(-) cells in ex vivo culture during retroviral transduction without a corresponding increase in SCID repopulating cell (SRC) frequency: Dissociation of SRC phenotype and function
5. High-efficiency retroviral transduction of mammalian cells on positively charged surfaces
6. Efficient retrovirus-mediated gene transfer to transplantable human BM cells in the absence of fibronectin
7. Highly efficient gene transfer into cord blood nonobese diabetic/severe combined immunodeficiency repopulating cells by oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (RD 114) envelope protein
8. High levels of lymphoid expression of enhanced green fluorescent protein in nonhuman primates transplanted with cytokine-mobilized peripheral blood CD34(+) cells
9. Gene therapy of human severe combined immunodeficiency (SCID)-X1 disease
10. + cells that mediate long-term engraftment of NOD/SCID mice by HIV vectors
11. Lentiviral vectors - The promise of gene therapy within reach?
12. Stem cell directed gene therapy
13. Lentiviral vectors: Turning a deadly foe into a therapeutic agent
14. Development of lentiviral vectors for gene therapy for human diseases
15. 1 human hematopoietic stem cells
16. Stable transduction of quiescent CD34(+)CD38(-) human hematopoietic cells by HIV-1-based lentiviral vectors
17. - cell transduction via lentivirus-based gene transfer vectors
18. Transduction of human progenitor hematopoietic stem cells by human immunodeficiency virus type 1-based vectors is cell cycle dependent
19. HIV-1 regulatory/accessory genes: Keys to unraveling viral and host cell biology
20. Cutting edge: A short polypeptide domain of HIV-1-Tat protein mediates pathogenesis
21. HIV-1 Tat inhibits human natural killer cell function by blocking L-type calcium channels
22. A murine leukemia virus (MuLV) long terminal repeat derived from rhesus macaques in the context of a lentivirus vector and MuLV gag sequence results in high-level gene expression in human T lymphocytes
23. Developmental analysis of the cytomegalovirus enhancer in transgenic animals
24. Marking and gene expression by a lentiviral vector in transplanted human and nonhuman primate CD34(+) cells
25. A GFP reporter system to assess gene transfer and expression in viable human hematopoietic progenitor cells
26. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector
27. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo
28. Generation of retroviral vector for clinical studies using transient transfection
29. Mature human hematopoietic cells in donor bone marrow complicate interpretation of stem/progenitor cell assays in xenogeneic hematopoietic chimeras
30. Quantity and quality of engrafting cells in cord blood and autologous mobilized peripheral blood
31. Recombinant retroviruses pseudotyped with the vesicular stomatitis virus G glycoprotein mediate both stable gene transfer and pseudotransduction in human peripheral blood lymphocytes
32. Critical factors influencing stable transduction of human CD34(+) cells with HIV-1-derived lentiviral vectors
33. One-day ex vivo culture allows effective gene transfer into human nonobese diabetic/severe combined immune-deficient repopulating cells using high-titer vesicular stomatitis virus G protein pseudotyped retrovirus
34. Consistent, persistent expression from modified retroviral vectors in murine hematopoietic stem cells
35. Separation of pluripotent haematopoietic stem cells from spleen colony-forming cells
36. Alternatives to stem cell renewal from a developmental viewpoint
37. Sustained, retransplantable, multilineage engraftment of highly purified adult human bone marrow stem cells in vivo
38. Distinct classes of human stem cells differ in proiliferaive and self-renewal potential
39. - BM and cord blood-derived SCID-repopulating cells with third-generation lentiviral vectors
40. Lentiviral vectors for enhanced gene expression in human hematopoietic cells
41. High-level transgene expression in human hematopoietic progenitors and differentiated blood lineages after transduction with improved lentiviral vectors
42. Lentiviral gene transfer into primary and secondary NOD/SCID repopulating cells