Conditions for single-strand conformation polymorphism (SSCP) analysis of BRCA1 gene using an automated electrophoresis unit

Clinical Chemistry and Laboratory Medicine

Volumn 39, Issue 5, 2001, Pages 401-404

  Campos, B ^a   Diez O ^a   Cortes J ^a   Domenech M ^a   Pericay, C ^a   Alonso, C ^a   Baiget, M ^a  

^a HOSPITAL DE LA SANTA CREU I SANT PAU   (Spain)

Author keywords

BRCA1 gene; Breast cancer; SSCP automated analysis

Indexed keywords

BRCA1 PROTEIN; DNA; DNA FRAGMENT; ICE; POLYACRYLAMIDE GEL; PRIMER DNA;

ARTICLE; AUTOMATION; CONTROLLED STUDY; DEVICE; DNA CONTENT; DNA DENATURATION; DNA TEMPLATE; ELECTROPHORETIC MOBILITY; FREEZING; GENE MUTATION; GENETIC ANALYSIS; GENETIC SCREENING; HUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM; TEMPERATURE;

BRCA1 PROTEIN; BREAST NEOPLASMS; DNA MUTATIONAL ANALYSIS; DNA PRIMERS; DNA, NEOPLASM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; GENES, BRCA1; GENETIC SCREENING; HUMANS; OVARIAN NEOPLASMS; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; TEMPERATURE;

EID: 0034993848     PISSN: 14346621     EISSN: None     Source Type: Journal    
DOI: 10.1515/CCLM.2001.064     Document Type: Article

References (10)

1. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction
2. Optimization of the single-strand conformation polymorphism (SSCP) technique for detection of point mutations
3. Optimization of single-strand conformation polymorphism analysis in the presence of polyethylene glycol
4. Breast and ovarian cancer incidence in BRCA1 mutation carriers
5. Rapid detection of BRCA1 mutations by the protein truncation test
6. Confirmation of BRCA1 analysis of germline mutations linked to breast and ovarian cancer in ten families
7. PCR conditions and DNA denaturants affect reproducibility of single-strand conformation polymorphism patterns for BRCA1 mutations
8. How sensitive is PCR-SSCP?
9. The sensitivity of single-strand conformation polymorphism analysis for the detection of single base substitutions