The crystal structure of E. Coli pantothenate synthetase confirms it as a member of the cytidylyltransferase superfamily

Structure

Volumn 9, Issue 5, 2001, Pages 439-450

  Von Delft, Frank ^a,d   Lewendon, Ann ^b   Dhanaraj, Venugopal ^a   Blundell, Tom L ^a   Abell, Chris ^c   Smith, Alison G ^a  

^a Department of Biochemistry ^*  (United Kingdom)

^b Pantherix West of Scotland Science Park   (United Kingdom)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^d SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Adenylate intermediate; Hinge bending; Rossmann fold; Selenomethionine MAD experiment; Vitamin B5

Indexed keywords

ADENOSINE TRIPHOSPHATE; AMINO ACID TRANSFER RNA LIGASE; CYTIDYLYLTRANSFERASE; PANTOTHENATE SYNTHETASE; PANTOTHENIC ACID; PEPTIDE SYNTHASE; RECOMBINANT ENZYME; SELENOMETHIONINE; TRANSFERASE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME PURIFICATION; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FOLDING; STRUCTURE ANALYSIS;

ADENOSINE TRIPHOSPHATE; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; ESCHERICHIA COLI; GENE EXPRESSION; PEPTIDE SYNTHASES; PROTEIN STRUCTURE, SECONDARY; SOLUTIONS; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0034984196     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(01)00604-9     Document Type: Article

References (54)

1. Biosynthesis of riboflavin, folic acid, thiamine, and pantothenic acid
2. The biosynthesis of pantothenic acid
3. Über pantothensäuresynthetase aus E. coli. V. Pantoyladenylat als acylierende komponente bei der enzymatischen synthese der pantothensäure
4. Pantothenate synthetase from Escherichia coli
5. β-alanine synthesis in Esherichia coli
6. Genetic and biochemical analysis of pantothenate biosynthesis in Escherichia coli and Salmonella typhimurium
7. Characterization and sequence of the Escherichia coli panBCD gene cluster
8. The final step of pantothenate biosynthesis in higher plants: Cloning and characterization of pantothenate synthetase from Lotus japonicus and Oryza sativum (rice)
9. The cytidylyltransferase superfamily: Identification of the nucleotide-binding site and fold prediction
10. Conserved cysteine and histidine residues in the structures of the tyrosyl and methionyl transfer RNA synthetases
11. Partition of transfer-RNA synthetases into 2 classes based on mutually exclusive sets of sequence motifs
12. Structure of tyrosyl transfer-RNA synthetase refined at 2.3 Å resolution-interaction of the enzyme with the tyrosyl adenylate intermediate
13. Evolutionary and structural relationships among dehydrogenases
14. Reactivity of selenomethionine - dents in the magic bullet?
15. The design and implementation of SnB version 2.0
16. Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods
17. Bias reduction in phase refinement by modified interference functions: Introducing the gamma correction
18. Bayesian statistical viewpoint on structure determination: Basic concepts and examples
19. Asparagine and glutamine rotamers: B-factor cutoff and correction of amide flips yield distinct clustering
20. OOPS-a-daisy. Joint CCP4 ESF-EACBM Newslett
21. PROCHECK - a program to check the stereochemical quality of protein structures
22. Errors in protein structures
23. The anatomy and taxonomy of protein structure
24. Touring protein fold space with Dali/FSSP
25. Architectures of class-defining and specific domains of glutamyl-tRNA synthetase
26. Structural basis for RNA aminoacylation by E. coli glutaminyl-tRNA synthetase
27. The crystal structure of a novel bacterial adenylyltransferase reveals half of sites reactivity
28. A prototypical cytidylyltransferase: CTP:glycerol-3-phosphate cytidylyltransferase from Bacillus subtilis
29. Sequence similarities among the family of aminoacyl transfer RNA synthetases
30. Involvement of threonine 234 in catalysis of tyrosyl adenylate formation by tyrosyl-tRNA synthetase
31. Validation of the general purpose TRIPOS 5.2 force field
32. The development of version 3 and version 4 of the Cambridge Structural Database System
33. Protein conformation-hinge bending and folding
34. Methionyl-tRNA synthetase needs an intact and mobile 332KMSKS336 motif in catalysis of methionyl adenylate formation
35. A crystal structure of ligand-free tryptophanyl-tRNA synthetase: Domain movements fragment the adenine nucleotide-binding site
36. How glutaminyl-tRNA synthetase selects glutamine
37. A possible prebiotic synthesis of pantetheine, a precursor to coenzyme A
38. Atomic structures of the human immunophilin FKBP-12 complexes with FK506 and rapamycin
39. Mounting of crystals for macromolecular crystallography in a freestanding thin-film
40. Data reduction
41. The CCP4 suite: Programs for protein crystallography
42. Statistical expectation value of the Debye-Waller factor and E(hkl) values for macromolecular crystals
43. Improved methods for building protein models in electron density maps and the location of errors in these models
44. Refinement of macromolecular structures by the maximum-likelihood method
45. Improved Fourier coefficients for maps using phases from partial structures with errors
46. Automated protein model building combined with iterative structure refinement
47. TNT refinement package
48. Recognition of spatial motifs in protein structures
49. JOY: Protein sequence-structure representation and analysis
50. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
51. Improved R factors for diffraction data analysis in macromolecular crystallography