Using immunohistochemistry to study plant metabolism: The examples of its use in the localization of amino acids in plant tissues, and of phosphoenolpyruvate carboxykinase and its possible role in pH regulation

Journal of Experimental Botany

Volumn 52, Issue 356, 2001, Pages 565-576

  Walker, Robert P ^a   Chen, Zhu Hui ^a   Johnson, Karen E ^a   Famiani, Franco ^b   Tecsi, Laszlo ^a   Leegood, Richard C ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b UNIVERSITY OF PERUGIA   (Italy)

Author keywords

Amino acid localization; Immunohistochemistry; Nitrogen metabolism; pH regulation; Phosphoenolpyruvate carboxykinase

Indexed keywords

AMINO ACIDS; ENZYMES; HISTOLOGY; IMMUNOLOGY; METABOLISM; NITROGEN; PH EFFECTS; PHYSIOLOGY; TISSUE;

IMMUNOHISTOCHEMISTRY;

PLANTS (BOTANY);

EID: 0034978567     PISSN: 00220957     EISSN: None     Source Type: Journal    
DOI: 10.1093/jxb/52.356.565     Document Type: Conference Paper

References (79)

1. Glycine-like immunoreactivity in the rat auditory pathway
2. Formation of sucrose from malate in germinating castor beans. I. Conversion of malate to phosphoenolpyruvate
3. Gluconeogenesis and ammoniagenesis in rat kidney: The effect of 3-mercaptopicolinic acid
4. Inducibility of Crassulacean acid metabolism (CAM) in Clusia species; physiological/biochemical characterisation and intercellular localization of carboxylation processes in three species which exhibit different degrees of CAM
5. -
6. Ammonium can stimulate nitrate and nitrite reductase in the absence of nitrate in Clematis vitalba
7. Involvement of HNF-1 in the regulation of phosphoenolpyruvate carboxykinase gene expression in kidney
8. Does phosphoenolpyruvate carboxykinase catalyse a gluconeogenic flux during the senescence of barley leaves or cucumber cotyledons?
9. Glutamate immunoreactivity in rat cerebral cortex is reversibly abolished by 6-diazo-5-oxo-L-norleucine (DON), an inhibitor of phosphate-activated glutaminase
10. The fine control of cytosolic pH
11. Phosphoenolpyruvate carboxykinase in plants exibiting Crassulacean acid metabolism
12. 4 plants
13. Ontogeny and structure of collenchyma and of vascular tissues in celery petioles
14. Phloem-specific expression of tyrosine/dopa decarboxylase genes and the biosynthesis of isoquinoline alkaloids in opium poppy
15. An immunohistochemical study of the compartmentation of metabolism during the development of grape (Vitis vinifera L.) berries
16. Tissue- and cell-specific expression of a cinnamyl alcohol dehydrogenase promoter in transgenic poplar plants
17. Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains
18. Microautoradiographic studies of phloem loading and transport in the leaf of Zea Mays L.
19. Intracellular pH regulation in maize root tips exposed to ammonium at high external pH
20. Relationship between intracellular pH and N metabolism in maize (Zea mays L.) roots
21. Biochemical and histochemical localization of monoterpene biosynthesis in the glandular trichomes of spearmint (Mentha spicata)
22. Phosphoenolpyruvate carboxykinase (GTP): The gene and the enzyme
23. Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression
24. 4 acid decarboxylating systems and ultrastructural features
25. Quantitative intercellular localization of NADH-dependent glutamate synthase protein in different types of root cells in rice plants
26. Promoter elements that mediate the pH response of PCK mRNA in LLC-PK1-F+ cells
27. Distribution and immunolocalization of stachyose synthase in Cucumis melo L.
28. Genomic and cDNA clones for maize phosphoenolpyruvate carboxylase and pyruvate, orthophosphate dikinase: Expression of different gene-family members in leaves and roots
29. Expression of NADH-dependent glutamate synthase protein in the epidermis and exodermis of rice roots in response to the supply of ammonium ions
30. Tissue-level localization of acid invertase in leaves and its potential for the regulation of carbon export
31. Invertase in leaves: Conundrum or control point?
32. Differential ammonia-elicited changes of cytosolic pH in root hair cells of rice and maize as monitored by 2′,7′-bis-(2-carboxyethyl)-5 (and-6)-carboxyfluorescein- fluorescence ratio
33. - on apoplast pH in the outer cortex of root zones of maize, as measured by the fluorescence ratio of fluorescein boronic acid
34. Macromolecular traffiking indicated by localization and turnover of sucrose transporters in enucleate sieve elements
35. Intracellular pH: Measurement and importance in cell activity
36. Cellular patterns of photosynthetic gene expression in developing maize leaves
37. Phosphoenolpyruvate carboxykinase in plants: Its role and regulation
38. The many-faceted function of phosphoenolpyruvate carboxykinase in plants
39. An immunohistochemical study of aspartate, glutamate and taurine in rat kidney
40. Effects of metabolic acidosis and diabetes on the abundance of specific renal mRNAs
41. Co-immunolocalization of topoisomerase II and chloroplast DNA in developing, dividing and mature wheat chloroplasts
42. The subcellular and intraorganelle recognition of nuclear and chloroplast transcripts in developing leaf cells
43. Cytoplasmic pH regulation in Acer pseudoplatanus cells. II. Possible mechanisms involved in pH regulation during acid-load
44. The use of microelectrodes to investigate compartmentation and the transport of metabolized inorganic ions in plants
45. Immunolocalization of a unique form of maize kernel glutamine synthetase using a monoclonal antibody
46. Amino acid and amide metabolism in the hulls and seeds of developing fruits of garden pea, Pisum sativum L. II. Asparagine
47. Glyoxylate cycle enzymes in seedlings and in mature plants of tomato (Lycopersicon esculentum Mill)
48. Banana sucrose-phosphate synthase gene expression during fruit ripening
49. Single-cell dissection and microdroplet chemistry
50. Enzyme immunocytochemistry
51. Induction of renal phosphoenolpyruvate carboxykinase mRNA: Suppressive effect of glucose
52. Acquisition of nitrogen by the shoots of land plants: Its occurrence and implications for acid-base regulation
53. Nitrogen assimilation and transport in vascular land plants in relation to intracellular pH regulation
54. The differential expression of sucrose synthase in relation to diverse patterns of carbon partitioning in developing cotton seed
55. Revision of biochemical pH-stat: Involvement of alternative pathway metabolisms
56. Degradation of endogenous organic acids induced by Pi uptake in Catharanthus roseus cells: Involvement of the biochemical pH-stat
57. Producing nitrate-free endive heads: Effect of nitrogen form on growth, yield and ion composition of endive
58. +. New aspects of an old problem
59. Gluconeogenesis from amino acids in germinating castor bean endosperm and its role in transport to the embryo
60. First visualization of glutamate and GABA in neurones by immunocytochemistry
61. Cellular compartmentation of ammonium
62. The pressure probe: A versatile tool in plant cell physiology
63. Cell sampling and analysis (SiCSA): Metabolites measured at single cell resolution
64. - uptake by roots of intact soybean plants
65. Changes in renal metabolite profile and ammoniagenesis during acute and chronic metabolic acidosis in dog and rat
66. Anatomy, chloroplast structure and compartmentation of enzymes relative to photosynthetic mechanisms in leaves and cotyledons of species in the tribe Salsoleae (Chenopodiaceae)
67. 4 plants: Its role and regulation
68. The role of cytosolic potassium and pH in the growth of barley roots
69. Phosphoenolpyruvate carboxykinase plays a role in interactions of carbon and nitrogen metabolism during grape seed development
70. Purification and phosphorylation in vivo and in vitro, of phosphoenolpyruvate carboxykinase from cucumber cotyledons
71. 4 photosynthesis and Crassulacean acid metabolism and in germinating seeds
72. Phosphoenolpyruvate carboxykinase from higher plants: Purification from cucumber and evidence of rapid proteolytic cleavage in extracts from a range of plant tissues
73. Hormonal and nutritional regulation of phosphoenolpyruvate carboxykinase mRNA levels in chicken kidney
74. Phosphoenolpyruvate carboxykinase is involved in the decarboxylation of aspartate in the bundle sheath of maize
75. Phloem transport of amino acids in relation to their cytosolic levels in barley leaves
76. Immunochemical localization of P-enolpyruvate carboxykinase in adult and developing mouse tissues
77. Chemical composition of apoplastic transport barriers in relation to radial hydraulic conductivity of corn roots (Zea mays L.)