Plasmid stability in a recombinant S cerevisiae strain secreting a bifunctional fusion protein

Journal of Chemical Technology and Biotechnology

Volumn 76, Issue 6, 2001, Pages 612-618

  Mete Altnta, M ^a   Krdar, Betul ^a   Lsen Onsan, Z ^a   Lgen, Kutlu O ^a  

^a BOGAZICI UNIVERSITY   (Turkey)

Author keywords

Bifunctional fusion protein; Plasmid copy number; Recombinant yeast; Segregational instability; Starch bioconversion

Indexed keywords

BIODEGRADATION; BIOREACTORS; ENZYMES; FERMENTATION; GENETIC ENGINEERING; PLASMA STABILITY; PROTEINS; STARCH;

BALANCE MODELS;

BIOTECHNOLOGY;

AMYLASE; GENE PRODUCT; GLUCAN 1,4 ALPHA GLUCOSIDASE; GLUCOSE; HYBRID PROTEIN; RECOMBINANT DNA; STARCH;

ANALYTIC METHOD; ARTICLE; ASPERGILLUS AWAMORI; BACILLUS SUBTILIS; BIOLOGICAL MODEL; BIOREACTOR; BIOTRANSFORMATION; CELL GROWTH; CELL POPULATION; CULTURE MEDIUM; FERMENTATION; KINETICS; MATHEMATICAL ANALYSIS; NONHUMAN; PLASMID; PREDICTION; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE;

ASPERGILLUS AWAMORI; BACILLUS SUBTILIS; SACCHAROMYCES CEREVISIAE;

EID: 0034975349     PISSN: 02682575     EISSN: None     Source Type: Journal    
DOI: 10.1002/jctb.424     Document Type: Article

References (14)

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9. Cloning of the α-amylase cDNA of Aspergillus shirousamii and its expression in Saccharomyces cerevisiae
10. Development of yeast strains for the efficient utilisation of starch: Evaluation of constructs that expresses α-amylase and glucoamylase separately or as bifunctional fusion proteins
11. Coexpression of a Saccharomyces diastaticus glucoamylase encoding gene and a Bacillus amyloliquefaciens α-amylase encoding gene in Saccharomyces cerevisiae
12. Ethanol production and fermentation characteristics of recombinant Saccharomyces cerevisiae strains grown on starch
13. A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding