Isolation, expression, and characterization of fully functional nontoxic BiP/GRP78 mutants

Protein Expression and Purification

Volumn 22, Issue 1, 2001, Pages 148-158

  King, LaShaunda ^a   Berg, Michael ^a   Chevalier, Mathieu ^a   Carey, Aileen ^a   Elguindi, Ebrahim C ^a   Blond, Sylvie Y ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

APOPTOSIS; CARBOXY TERMINAL SEQUENCE; CELL GROWTH; CHAPERONE; CYTOTOXICITY; DEPOLYMERIZATION; GENE OVEREXPRESSION; GENETIC CONSERVATION; GROWTH INHIBITION; HEAT SHOCK PROTEIN 70; MUTANT PROTEIN; PROTEIN BINDING; PROTEIN INDUCTION; PROTEIN PURIFICATION; SYNTHETIC PEPTIDE; WILD RELATIVE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA; MURINAE;

EID: 0034971343     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1424     Document Type: Article

References (51)

1. Physiological consequences of DnaK and DnaJ overproduction in Escherichia coli
2. Mutant membrane protein toxicity
3. Death effector domain of a mammalian apoptosis mediator, FADD, induces bacterial cell death
4. Familial mutations and the thermodynamic stability of the recombinant human prion protein
5. Multicopy plasmid suppression of stationary phase chaperone toxicity in E. Coli by phosphogluconate dehydratase and the N-terminus of DnaK
6. Peptide-dependent stimulation of the ATPase activity of the molecular chaperone BiP is the result of conversion of oligomers to active monomers
7. Disulphide bridge formation in the periplasm of Escherichia coli β-lactamase: Human IgG3 hinge fusions as a model system
8. Increased production of human proinsulin in the periplasmic space of Escherichia coli by fusion to DsbA
9. Compatible-solute-supported periplasmic expression of functional recombinant proteins under stress conditions
10. Folding of a mutant maltose-binding protein of Escherichia coli which forms inclusion bodies
11. Specific aggregation of partially folded polypeptide chains: The molecular basis of inclusion body composition
12. The specificity of protein aggregation
13. From Levinthal to pathways to funnels
14. Artificial chaperone-assisted refolding of citrate synthase
15. DnaK/DnaJ supplementation improves the periplasmic production of human granulocyte-colony stimulating factor in E. Coli
16. Cytoplasmic and periplasmic production of human placental glutathione transferase in Escherichia coli
17. Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing heat-shock proteins
18. Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm
19. Molecular chaperones stimulate the functional expression of the cocaine-sensitive serotonin transporter
20. Successive action of DnaK, DnaJ and GroEL along the pathway of chaperone-mediated protein folding
21. Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network
22. Recombinant toxins as novel therapeutic agents
23. Thermotolerant cells show an attenuated expression of Hsp70 after heat shock
24. BiP and Sec63p are required for both co- and posttranslational protein translocation into the yeast endoplasmic reticulum
25. Multiple molecular chaperone complex with misfolded large oligomeric glycoproteins in the endoplasmic reticulum
26. Multiple molecular chaperones interact with apolipoprotein B during its maturation
27. 2+ disturbances, and cell death in renal epithelial cells
28. Heavy chain binding protein recognizes incompletely disulfide-bonded forms of vesicular stomatitis virus G protein
29. Mutant analysis links the translocon and BiP to retrograde protein transport for ER degradation
30. The requirement for molecular chaperones during endoplasmic reticulum-associated protein degradation demonstrates that protein export and import are mechanistically distinct
31. Substrate binding induces depolymerization of the C-terminal peptide binding domain of murine GRP78/BiP
32. Specificity of peptide-induced depolymerization of the recombinant carboxy-terminal fragment of BiP/GRP78
33. Interaction of murine BiP/GRP78 with the DnaJ homologue MTJ1
34. Kinetic characterization of the ATPase cycle of the DnaK molecular chaperone
35. A PCR-based strategy for extensive mutagenesis of a target DNA sequence
36. Mapping important nucleotides in the peptidyl transferase centre of 23S rRNA using a random mutagenesis approach
37. Purification and properties of BiP
38. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
39. Calculation of protein extinction coefficients from amino acids sequence data
40. A bipartite signaling mechanism involved in DnaJ-mediated activation of the Escherichia coli DnaK protein
41. Mutations altering heat shock specific subunit of RNA polymerase suppress major cellular defects of E. Coli mutants lacking the DnaK chaperone
42. Three-dimensional structure of the ATPase fragment of a 70K heat-shock cognate protein
43. Identification of the peptide binding domain of hsc70: 18-Kilodalton fragment located immediately after ATPase domain is sufficient for high affinity binding
44. Structural analysis of substrate binding by the molecular chaperone DnaK
45. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
46. NMR solution structure of the 21 kDa chaperone protein DnaK substrate binding domain: A preview of chaperone-protein interaction
47. High-resolution solution structure of the 18 kDa substrate-binding domain of the mammalian chaperone protein hsc70
48. Sls1p stimulates Sec63p-mediated activation of Kar2p in a conformation-dependent manner in the yeast endoplasmic reticulum
49. Mutations in the C-terminal fragment of DnaK affecting peptide binding
50. Topology and dynamics of the 10 kDa C-terminal domain of DnaK in solution