Trypsin cleavage stabilizes the rotavirus VP4 spike

Journal of Virology

Volumn 75, Issue 13, 2001, Pages 6052-6061

  Crawford, S E ^a   Mukherjee, S K ^a   Estes, M K ^a   Lawton, J A ^a   Shaw, A L ^a   Ramig, R F ^a   Prasad, B V V ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRYPSIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; FETUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN STRUCTURE; ROTAVIRUS; SEROTYPE; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; VIRUS CAPSID; VIRUS INFECTIVITY; VIRUS MORPHOLOGY;

AMINO ACID SEQUENCE; ANIMALS; CAPSID; CAPSID PROTEINS; CERCOPITHECUS AETHIOPS; MICROSCOPY, ELECTRON; ROTAVIRUS; TRYPSIN;

EID: 0034968905     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.13.6052-6061.2001     Document Type: Article

References (53)

1. Trypsin activation pathway of rotavirus infectivity
2. Age dependent diarrhea induced by a rotaviral nonstructural glycoprotein
3. Characterization of G serotype dependent non-antibody inhibitors of rotavirus in normal mouse serum
4. Biological and immunological characterization of a simian rotavirus SA11 variant with an altered genome segment 4
5. Functional and topographical analyses of epitopes on the hemagglutinin (VP4) of the simian rotavirus SA11
6. Phenotypes of rotavirus reassortants depend upon the recipient genetic background
7. Rescue of infectivity by sequential in vitro transcapsidation of rotavirus core particles with inner capsid and outer capsid proteins
8. Determinants of rotavirus stability and density during CsCl purification
9. Human and most animal rotavirus strains do not require the presence of sialic acid on the cell surface for efficient infectivity
10. Characterization of neutralization epitopes on the VP7 surface protein of serotype G11 porcine rotaviruses
11. Rotavirus contains integrin ligand sequences and a disintegrin-like domain that are implicated in virus entry into cells
12. Characterization of virus-like particles produced by the expression of rotavirus capsid proteins in insect cells
13. Three-dimensional reconstruction and the architecture of spherical viruses
14. The reconstruction of a three-dimensional structure from projections and its application to electron microscopy
15. 2+ concentration to initiate their replication cycle
16. Cryo-electron microscopy of vitrified specimens
17. Rotaviruses and their replication
18. Proteolytic enhancement of rotavirus infectivity: Molecular mechanisms
19. Mapping the hemagglutination domain of rotaviruses
20. Role of VP3 in human rotavirus internalization after target cell attachment via VP7
21. The T=4 envelope of Sindbis virus is organized by interactions with a complementary T=3 capsid
22. Cleavage of Rhesus rotavirus VP4 after arginine 247 is essential for rotavirus-like particle-induced fusion from without
23. Integrins alpha2beta1 and alpha4beta1 can mediate SA11 rotavirus attachment and entry into cells
24. Functional and structural analysis of the sialic acid-binding domain of rotaviruses
25. Infectious rotavirus enters cells by direct cell membrane penetration, not by endocytosis
26. Acidification of endosomes is not important for the entry of rotavirus into the cell
27. Attachment and growth of human rotaviruses RV-3 and S12/85 in Caco-2 cells depend on VP4
28. Automated software package for icosahedral virus reconstruction
29. Three-dimensional structural analysis of recombinant rotavirus-like particles with intact and amino-terminal-deleted VP2: Implications for the architecture of the VP2 capsid layer
30. Productive penetration of rotavirus in cultured cells induces coentry of the translation inhibitor alfa-sarcin
31. Genetic mapping indicates that VP4 is the rotavirus cell attachment protein in vivo and in vitro
32. Rotavirus proteins VP7, NS28 and VP4 form oligomeric structures
33. Biochemical mapping of the simian rotavirus SA11 genome
34. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
35. Sequence of a rotavirus gene 4 associated with unique biological properties
36. Interactions between the two surface proteins of rotavirus may alter the receptor-binding specificity of the virus
37. Reproducible high yield sequencing of proteins electrophoretically separated and transferred to an inert support
38. Interaction of rotavirus particles with liposomes
39. Rotavirus-induced structural and functional alterations in tight junctions of polarized intestinal Caco-2 cell monolayers
40. Genomic heterogeneity of simian rotavirus SA11
41. Localization of VP4 neutralization sites in rotavirus by 3D cryo-electron microscopy
42. Molecular basis of rotavirus replication: Structure-function correlations
43. Electron cryomicroscopy and computer image processing techniques: Use in structure-function studies of rotavirus
44. Visualization of ordered genomic RNA and localization of transcriptional complexes in rotavirus
45. Three-dimensional structure of rotavirus
46. Cell lines susceptible to infection are permeabilized by cleaved and solubilized outer layer proteins of rotavirus
47. Preliminary characterization of an epitope involved in neutralization and cell attachment protein that is located on the major bovine rotavirus glycoprotein
48. Three-dimensional visualization of the rotavirus hemagglutinin structure
49. Similarity measures between images
50. Studies on the influence of the VP7 gene on rotavirus replication
51. Three-dimensional structure of the rotavirus haemagglutinin VP4 by cryo-electron microscopy and difference map analysis
52. 3D structure of rhesus rotavirus by cryoelectron microscopy and image reconstruction
53. Protein subunit structures in the herpes simplex virus A-capsid determined from 400 kV spot-scan electron cryomicroscopy