Biological and biochemical characterization of variant a subunits of cholera toxin constructed by site-directed mutagenesis

Journal of Bacteriology

Volumn 183, Issue 13, 2001, Pages 4024-4032

  Jobling, M G ^a   Holmes, R K ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLERA TOXIN; LIVE VACCINE;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; CYTOLOGY; DELETION MUTANT; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE CONSTRUCT; GENE INSERTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS; TOXICITY; TOXIN ANALYSIS; TOXIN STRUCTURE; VIBRIO CHOLERAE;

ADP-RIBOSYLATION FACTORS; AMINO ACID SEQUENCE; BACTERIAL TOXINS; BINDING SITES; CHOLERA TOXIN; CONSERVED SEQUENCE; ENTEROTOXINS; ENZYME STABILITY; EPITOPES; ESCHERICHIA COLI PROTEINS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; VARIATION (GENETICS);

EID: 0034968234     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.13.4024-4032.2001     Document Type: Article

References (50)

1. Structure of exotoxin A of Pseudomonas aeruginosa at 3.0 angstrom resolution
2. Crystal structure of diphtheria toxin bound to nicotinamide adenine dinucleotide
3. The crystal structure of diphtheria toxin
4. Site-directed mutagenic alteration of potential active-site residues of the A subunit of Escherichia coli heat-labile enterotoxin - Evidence for a catalytic role for glutamic acid 112
5. Role of tryptophan 26 in the NAD glycohydrolase reaction of the S-1 subunit of pertussis toxin
6. Functional comparison of the NAD binding cleft of ADP-ribosylating toxins
7. Three conserved consensus sequences identify the NAD-binding site of ADP-ribosylating enzymes, expressed by eukaryotes, bacteria and T-even bacteriophages
8. Stepwise transplantation of an active site loop between heat-labile enterotoxins LT-II and LT-I and characterization of the obtained hybrid toxins
9. Protein engineering studies of A-chain loop 47-56 of Escherichia coli heat-labile enterotoxin point to a prominent role of this loop for cytotoxicity
10. Binding of NAD+ by cholera toxin
11. The arrangement of subunits in cholera toxin
12. ADP-ribosylation of membrane proteins catalyzed by cholera toxin: Basis of the activation of adenylate cyclase
13. Mucosal adjuvanticity and immunogenicity of LTR72, a novel mutant of Escherichia coli heat-labile enterotoxin with partial knock-out of ADP-ribosyltransferase activity
14. Importance of ADP-ribosylation in the morphological changes of PC12 cells induced by cholera toxin
15. Mutants of cholera toxin as an effective and safe adjuvant for nasal influenza vaccine
16. Heat-labile enterotoxins (Escherichia coli)
17. Cholera toxin and related enterotoxins of Gram-negative bacteria
18. Characterization of monoclonal antibodies that react with unique and cross-reacting determinants of cholera enterotoxin and its subunits
19. Analysis of structure and function of the B subunit of cholera toxin by the use of site directed mutagenesis
20. Fusion proteins containing the A2 domain of cholera toxin assemble with B polypeptides of cholera toxin to form immunoreactive and functional holotoxin-like chimeras
21. Identification of motifs in cholera toxin A1 polypeptide that are required for its interaction with human ADP-ribosylation factor 6 in a bacterial two-hybrid system
22. Construction and characterization of versatile cloning vectors for efficient delivery of native foreign proteins to the periplasm of Escherichia coli
23. 1 peptide and activation of adenylate cyclase
24. Histidine-44 of the A subunit of Escherichia coli enterotoxin is involved in its enzymatic and biological activities
25. Rapid and efficient site specific mutagenesis without phenotypic selection
26. Determination of the kinetic mechanism of arginine-specific ADP-ribosyltransferases using a high performance liquid chromatographic assay
27. Effect of site-directed mutagenic alterations on ADP-ribosyltransferase activity of the A subunit of Escherichia coli heat-labile enterotoxin
28. A proposed mechanism of ADP-ribosylation catalyzed by the pertussis toxin S1 subunit
29. Mutations in the A subunit affect yield, stability, and protease sensitivity of nontoxic derivatives of heat-labile enterotoxin
30. A tissue culture method for the detection of bacterial enterotoxins
31. Enzymatic activity of cholera toxim I. New method of assay and the mechanism of ADP-ribosyl transfer
32. Enzymatic activity of cholera toxin. II. Relationships to proteolytic processing, disulfide bond reduction and subunit composition
33. Effects of temperature on ADP-ribosylation factor stimulation of cholera toxin activity
34. Assay of heat-labile enterotoxins by their ADP-ribosyltransferase activities
35. Kinetic mechanism of two NAD:arginine ADP-ribosyltransferases: The soluble, salt-stimulated transferase from turkey erythrocytes and choleragen, a toxin from Vibrio cholerae
36. An arabinose-inducible expression vector, pAR3, compatible with ColE1-derived plasmids
37. Mutants of pertussis toxin suitable for vaccine development
38. Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis
39. + hydrolysis by the cholera toxin catalytic subunit
40. Refined structure of Escherichia coli heat-labile enterotoxin, a close relative of cholera toxin
41. Crystal structure of a cholera toxin-related heat-labile enterotoxin of Escherichia coli
42. The crystal structure of pertussis toxin
43. Protein phosphorylation and regulation of adaptive responses in bacteria
44. A bacteriophage T7 RNA polymerase/promoter system for the controlled exclusive expression of specific genes
45. Improved media for growing plasmid and cosmid clones
46. Effective mucosal immunization against respiratory syncytial virus using purified F protein and a genetically detoxified cholera holotoxin, CT-E29H
47. The Arg7 Lys mutant of heat-labile enterotoxin exhibits great flexibility of active site loop 47-56 of the A subunit
48. Crystal structure of a new heat-labile enterotoxin, LT-IIb
49. ADP-ribosylation factors: A family of guanine nucleotide-binding proteins that activate cholera toxin and regulate vesicular transport
50. The three-dimensional crystal structure of cholera toxin