The effect of prefreezing the diluent portion of the straw in a step-wise vitrification process using ethylene glycol and polyvinylpyrrolidone to preserve bovine blastocysts

Cryobiology

Volumn 42, Issue 2, 2001, Pages 135-138

  Mtango, N R ^a   Varisanga, M D ^a   Dong, Y J ^a   Otoi, T ^a   Suzuki, T ^a  

^a YAMAGUCHI UNIVERSITY   (Japan)

Author keywords

Blastocysts; In straw dilution; Prefrozen straw; Vitrification

Indexed keywords

CRYOPROTECTIVE AGENT; ETHYLENE GLYCOL; GENTAMICIN; INSULIN; LIQUID NITROGEN; POVIDONE; TREHALOSE; WATER;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BATH; BLASTOCYST; CELL CULTURE; CELL SURVIVAL; CONTROLLED STUDY; CRYOPROTECTION; DILUTION; EMBRYO; FERTILIZATION; FREEZING; HATCHING; IMMERSION; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE DEPENDENCE; THAWING;

EID: 0034964534     PISSN: 00112240     EISSN: None     Source Type: Journal    
DOI: 10.1006/cryo.2001.2308     Document Type: Article

References (11)

1. Vitrification as an approach to cryopreservation
2. Survival of IVF-derived bovine embryos frozen in glycerol or ethylene glycol
3. Ultrastructure of IVM-IVF ovine blastocysts vitrified after equilibration in glycerol, 1,2-propanediol using 2-step and 16-step procedures
4. Containerless vitrification of mammalian oocytes and embryos
5. Development into blastocyst of bovine oocytes cryopreserved by ultra-rapid cooling
6. Ultrastructure of bovine in vitro produced blastocysts cryopreserved by vitrification
7. Effects of vitrification medium composition on the survival of bovine in vitro produced embryos, following in straw-dilution, in vitro and in vivo following transfer
8. Factors affecting the survival of mouse embryos cryopreserved by vitrification
9. Viability of bovine blastocysts obtained after 7, 8, or 9 days of culture in vitro following vitrification and one-step rehydration
10. Open pulled straw (OPS) vitrification: A new way to reduce cryoinjuries of bovine ova and embryos
11. The effect of media, serum and temperature on in vitro survival of bovine blastocysts after open pulled straw (OPS) vitrification