Constructing cDNA libraries with fewer clones that contain long poly(dA) tails

BioTechniques

Volumn 31, Issue 1, 2001, Pages 38-42

  Jiang, H ^a   Bivens, N J ^a   Ries, J E ^a   Whitworth, K M ^a   Green, J A ^a   Forrester, L J ^a   Springer, G K ^a   Didion, B A ^a   Mathialagan, N ^a   Prather, R S ^a   Lucy, M C ^a  

^a UNIVERSITY OF MISSOURI   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA;

ARTICLE; DNA LIBRARY; DNA SEQUENCE; DNA SYNTHESIS; EXPRESSED SEQUENCE TAG; GENE CONSTRUCT; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION; TECHNIQUE; TEMPERATURE;

EID: 0034960349     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01311bm05     Document Type: Article

References (6)

1. Complementary DNA sequencing: Expressed sequence tags and human genome project
2. Generation and analysis of 280,000 human expressed sequence tags
3. Efficient double stranded sequencing of cDNA clones containing long poly(A) tails using anchored poly(dT) primers
4. Sequencing of 3′ cDNA clones using anchored oligo(dT) primers
5. Construction and screening of normalized cDNA libraries