Assessment of benzimidazole binding to individual recombinant tubulin isotypes from Haemonchus contortus

Parasitology

Volumn 122, Issue 6, 2001, Pages 683-687

  Oxberry, M E ^a,d   Geary, T G ^c   Prichard, R K ^b  

^a CURTIN UNIVERSITY   (Australia)

^b MCGILL UNIVERSITY   (Canada)

^c Animal Health Discovery Research   (United States)

^d Molecular Immunology Laboratory   (Australia)

Author keywords

Benzimidazole; Haemonchus contortus; Mebendazole; Receptor ligand interactions; Tubulin

Indexed keywords

ALPHA TUBULIN; BENZIMIDAZOLE; BETA TUBULIN; DRUG RECEPTOR; MEBENDAZOLE; RECOMBINANT PROTEIN; TUBULIN;

ARTICLE; BINDING AFFINITY; DRUG PROTEIN BINDING; ESCHERICHIA COLI; HAEMONCHUS CONTORTUS; MICROTUBULE; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; STOICHIOMETRY;

ANIMALS; ANTHELMINTICS; BINDING SITES; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HAEMONCHIASIS; HAEMONCHUS; HELMINTH PROTEINS; KINETICS; MEBENDAZOLE; MICROTUBULES; PROTEIN ISOFORMS; RECOMBINANT PROTEINS; TUBULIN;

ESCHERICHIA COLI; HAEMONCHUS CONTORTUS; NEMATODA;

EID: 0034958718     PISSN: 00311820     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0031182001007788     Document Type: Article

References (25)

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3. Genetic variability of the beta-tubulin genes in benzimidazole-susceptible and -resistant strains of Haemonchus contortus
4. 2+ dependence of guanine nucleotide binding to tubulin
5. Photoaffinity labelling of tubulin with (2-nitro-4-azidophenyl) deacetylcolchicine: Direct evidence for two colchicine binding sites
6. Three β-tubulin cDNAs from the parasitic nematode Haemonchus contortus
7. The kinetics of mebendazole binding to Haemonchus contortus tubulin
8. Identification of an amino acid substitution in the ben A, β-tubulin gene of Aspergillus nidulans that confers thiabendazole resistance and benomyl supersensitivity
9. Antiprotozoal activities of benzimidazoles and correlations with β-tubulin sequence
10. Effect of selection for benzimidazole resistance in Haemonchus contortus on β-tubulin isotype 1 and isotype 2 genes
11. The role of the cytoskeletal protein, tubulin, in the mode of action and mechanisms of drug resistance to benzimidazoles
12. Assessment of microtubule stabilizers by semiautomated in vitro microtubule protein polymerization and mitotic block assays
13. Expression of cloned β-tubulin genes of Haemonchus contortus in Escherichia coli: Interaction of recombinant β-tubulin with native tubulin and mebendazole
14. Haemonchus contortus: The role of two beta-tubulin gene subfamilies in the resistance to benzimidazole anthelmintics
15. Beta-tubulin and benzimidazole resistance in the sheep nematode Haemonchus contortus
16. Specific interaction of benzimidazole anthelmintics with tubulin: High affinity binding and benzimidazole resistance in Haemonchus contortus
17. Structure-activity relationships of benzothiazole and benzimidazole anthelmintics: A molecular modelling approach to in vivo drug efficacy
18. Individual expression of recombinant α- and β-tubulin from Haemonchus contortus: Polymerisation and drug effects
19. Local unfolding and the stepwise loss of the functional properties of tubulin
20. Molecular mechanism of colchicine action: Induced local unfolding of β-tubulin
21. Involvement of the carboxy-terminal domain in the regulation of its assembly
22. Localization of the colchicine binding site of tubulin
23. Preparation of tubulin from brain
24. Direct photoaffinity labelling of tubulin with colchicine