Expression of the amino-terminal domain of platelet glycoprotein ibα: Exploitation of a calmodulin tag for determination of its functional activity

Protein Expression and Purification

Volumn 22, Issue 2, 2001, Pages 200-210

  Li, Chester Q ^a   Ye, Pei ^a   Cao, Zhao Feng ^a   Wang, Huifang ^a   Lu, Lan ^a   Nicastro, Peter ^a   Wood, Emma ^c   Robert, Justin J ^a   Ouwehand, Willem H ^c,d   Hill, Fergal ^c   López, José A ^e   Wardell, Mark R ^a,b  

^a Department of Biochemistry and Molecular Biophysics ^*  (United States)

^b WASHINGTON UNIVERSITY   (United States)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^d NHS BLOOD AND TRANSPLANT   (United Kingdom)

^e BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO TERMINAL SEQUENCE; BINDING SITE; CALMODULIN; CULTURE MEDIUM; GLYCOPROTEIN IB; HYBRID PROTEIN; INSECT CELL; LIGAND BINDING; MOLECULAR INTERACTION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PURIFICATION; THROMBIN; VON WILLEBRAND FACTOR;

INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0034957928     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1441     Document Type: Article

References (52)

1. Biochemistry and genetics of von Willebrand factor
2. The platelet glycoprotein Ib-IX complex
3. Molecular mechanisms of platelet adhesion and activation
4. The platelet glycoprotein Ib-IX complex
5. Role of glycoprotein V in the formation of the platelet high-affinity thrombin binding site
6. Expression of platelet glycoprotein (GP) V in heterologous cells and evidence for its association with GP Ibα in forming a GP Ib-IX-V complex on the cell surface
7. The glycocalicin portion of platelet glycoprotein Ib expresses both high and moderate affinity receptor sites for thrombin. A soluble radioreceptor assay for the interaction of thrombin with platelets
8. Platelet glycoprotein Ibα binds to thrombin anion-binding exosite II inducing allosteric changes in the activity of thrombin
9. The von Willebrand factor-binding domain of platelet membrane glycoprotein Ib. Characterization by monoclonal antibodies and partial amino acid sequence analysis of proteolytic fragments
10. Platelet glycocalicin. Interaction with thrombin and role as thrombin receptor of the platelet surface
11. 293of the α-chain of glycoprotein Ib
12. Identification of a site in the α chain of platelet glycoprotein Ib that participates in von Willebrand factor binding
13. Structure and function of platelet membrane glycoproteins Ib and V. Effects of leukocyte elastase and other proteases on platelets response to von Willebrand factor and thrombin
14. Tyrosine sulfation of the glycoprotein Ib-IX complex: Identification of sulfated residues and effect on ligand binding
15. Identification of three tyrosine residues of glycoprotein Ibα with distinct roles in von Willebrand factor and α-thrombin binding
16. Bernard-Soulier syndrome
17. Platelet-type von Willebrand's disease: Characterization of a new bleeding disorder
18. Mutation in the gene encoding the α chain of platelet glycoprotein Ib in platelet-type von Willebrand disease
19. Pseudo-von Willebrand disease: A mutation in the platelet glycoprotein Ibα gene associated with a hyperactive surface receptor
20. Further characterization of platelet-type von Willebrand's disease in Japan
21. Substitution of Val for Met at residue 239 of platelet glycoprotein Ibα in Japanese patients with platelet-type von Willebrand disease
22. Pseudo-von Willebrand's disease. An intrinsic platelet defect with aggregation by unmodified human factor VIII/von Willebrand factor and enhanced absorption of its high molecular-weight multimers
23. Molecular biology for platelet alloantigen typing
24. Localization of the platelet-specific HPA-2 (Ko) alloantigens on the N-terminal globular fragment of platelet glycoprotein Ibα
25. 145 amino acid polymorphism, which is associated with the HPA-2(Ko) alloantigens
26. Genetic and structural characterization of an amino acid dimorphism in glycoprotein Ibα involved in platelet transfusion refractoriness
27. Expression and characterization of functionally active fragments of the platelet glycoprotein (GP) Ib-IX complex in mammalian cells
28. Site-directed mutagenesis of a soluble recombinant fragment of platelet glycoprotein Ibα demonstrating negatively charged residues involved in von Willebrand factor binding
29. Transient expression of recombinant glycoprotein Ibα polypeptides in COS cells that inhibit von Willebrand factor binding to the platelet glycoprotein Ib/IX complex
30. Stable expression in Chinese hamster ovary cells of a homogeneous recombinant active fragment of human platelet glycoprotein Ibα
31. Identification of the disulphide bonds in human platelet glycocalicin
32. Protein measurement with the Folin phenol reagent
33. Efficient plasma membrane expression of a functional platelet glycoprotein Ib-IX complex requires the presence of its three subunits
34. Calmodulin as a versatile tag for antibody fragments
35. Calcium-dependent affinity chromatography of S-100 and calmodulin on calmodulin antagonist-coupled Sepharose
36. Glycosylation inhibitors for N-linked glycoproteins
37. Effect of tunicamycin on the synthesis of macromolecules in cultures of chick embryo fibroblasts infected with Newcastle disease virus
38. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
39. Alanine substitutions in calmodulin-binding peptides result in unexpected affinity enhancement
40. Engineering N-glycosylation pathways in the baculovirus-insect cell system
41. Aggregation of mammalian cells expressing the platelet glycoprotein (GP) Ib-IX complex and the requirement for tyrosine sulfation of GP Ibα
42. Interaction of von Willebrand factor domain A1 with platelet glycoprotein Ibα-(1-289). Slow intrinsic binding kinetics mediate rapid platelet adhesion
43. Hydrolysis of human platelet membrane glycoproteins with a Serratia rnarcescens metalloprotease: Effect on response to thrombin and von Willebrand factor
44. Platelet glycocalicin: A single receptor for platelet aggregation induced by thrombin or ristocetin
45. Platelet glycoprotein Ibα is a counterreceptor for the leukocyte integrin Mac-1 (CD11b/CD18)
46. Functional analysis of a recombinant glycoprotein Ibα polypeptide which inhibits von Willebrand factor binding to the platelet glycoprotein Ib-IX complex and to collagen
47. Signals important for high-level expression of foreign genes in Autographa californica nuclear polyhedrosis virus expression vectors
48. Baculoviruses: High level expression in insect cells
49. Expression of recombinant baculovirus for vitronectin in insect cells: Purification, characterization of post-translational modifications and functional studies of the recombinant protein
50. Amino acid sequence of the von Willebrand factor-binding domain of platelet membrane glycoprotein Ib
51. 2-glycoprotein
52. Threonine-145/methionine-145 variants of baculovirus-produced recombinant ligand binding domain of GPIbα express HPA-2 epitopes and show equal binding of von Willebrand factor