Expression and purification of cytokine receptor homology domain of human granulocyte-colony-stimulating factor receptor fusion protein in Escherichia coli

Protein Expression and Purification

Volumn 21, Issue 1, 2001, Pages 87-91

  Tatsuda, Daisuke ^a   Arimura, Haruhiko ^a   Tokunaga, Hiroko ^a   Ishibashi, Matsujiro ^a   Arakawa, Tsutomu ^b   Tokunaga, Masao ^a  

^a KAGOSHIMA UNIVERSITY   (Japan)

^b ALLIANCE PROTEIN LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CYTOKINE RECEPTOR; ESCHERICHIA COLI; GLUTATHIONE TRANSFERASE; GRANULOCYTE COLONY STIMULATING FACTOR; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; THIOREDOXIN;

EID: 0034957034     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1343     Document Type: Article

References (19)

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7. Functional domains of the granulocyte colony-stimulating factor receptor
8. Expression and purification of cytokine receptor homology domain of human granulocyte-colony stimulating factor receptor in Escherichia coli
9. Gene fusion expression systems in Escherichia coli
10. Using PCR to engineer DNA
11. DNA sequencing with chain-terminating inhibitors
12. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
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15. Protein measurement with the Folin-phenol reagent
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17. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm
18. Formation of 1:1 complex of the cytokine receptor homologous region of granulocyte colony-stimulating factor receptor with ligand
19. Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli