Increased yield of PCR products by addition of T4 gene 32 protein to the SMART™ PCR cDNA synthesis system

BioTechniques

Volumn 31, Issue 1, 2001, Pages 81-86

  Villalva, C ^a   Touriol, C ^a   Seurat, P ^a   Trempat, P ^a   Delsol, G ^a   Brousset, P ^a  

^a CHU PURPAN   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ENZYMES; GENES; RNA; SYNTHESIS (CHEMICAL); TISSUE;

REVERSE TRANSCRIPTASE;

PROTEINS;

COMPLEMENTARY DNA; MESSENGER RNA; PROTEIN; T4 GENE 32 PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL LEVEL; DEVICE; DNA SYNTHESIS; EVALUATION; HUMAN; HUMAN CELL; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TECHNIQUE;

EID: 0034953559     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01311st04     Document Type: Article

References (12)

1. A coupled one-step reverse transcription PCR procedure for generation of full-length open reading frames
2. Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR
3. Specific association of human telomerase activity with immortal cells and cancer
4. New specificity and yield enhancer of polymerase chain reactions
5. Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein
6. Reverse transcriptase motifs in the catalytic subunit of telomerase
7. Quantification of mRNA by non-radioactive RT-PCR and CDD imaging system
8. Protein-protein interactions at a DNA replication fork: Bacteriophage T4 as a model
9. Effects of the T4 bacteriophage gene 32 product on the efficiency and fidelity of DNA amplification using T4 DNA polymerase
10. Improved yields of long PCR products using gene 32 protein
11. Reverse transcriptase inhibits Taq polymerase activity
12. T4 phage gene 32 protein as a candidate organizing factor for the deoxyribonucleoside triphosphate synthetase complex