Purification and characterization of a Z-Pro-prolinal-insensitive Z-Gly-Pro-7-amino-4-methyl coumarin-hydrolyzing peptidase from bovine serum - A new proline-specific peptidase

Protein Expression and Purification

Volumn 22, Issue 2, 2001, Pages 286-298

  Birney, Yvonne A ^a   O'Connor, Brendan F ^a  

^a DUBLIN CITY UNIVERSITY   (Ireland)

Author keywords

PO; Substrate specificity; Z Gly Pro MCA; Z Pro prolinal; ZIP

Indexed keywords

AMINO ACID; CHROMATOGRAPHY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; FATTY ACID BINDING PROTEIN; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; ISOELECTRIC POINT; MOLECULAR WEIGHT; PEPTIDASE; PH; PROLINE; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS; SERINE PROTEINASE; SPECIFICITY;

BOVINAE;

EID: 0034951622     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1450     Document Type: Article

References (41)

1. Leucylglycinamide released from oxytocin by human uterine enzyme
2. Post proline cleaving enzyme: Synthesis of a new fluorogenic substrate and distribution of the endopeptidase in rat tissues and body fluids of man
3. cDNA cloning of porcine brain prolyl endopeptidase and identification of the active-site seryl residue
4. The purification, characterization and analysis of primary and secondary-structure of prolyl oligopeptidase from human lymphocytes. Evidence that the enzyme belongs to the α/β hydrolase fold family
5. A study of prolyl endopeptidase in bovine serum and its relevance to the tissue enzyme
6. Identification and initial characterisation of a N-benzyloxycarbonyl-prolyl-prolinal (Z-Pro-prolinal)-insensitive 7-(N-benzyloxycarbonyl-glycyl-prolyl-amido)-4-methylcoumarin (Z-Gly-Pro-NH-Mec)-hydrolysing peptidase in bovine serum
7. Molecular cloning and characterization of prolyl endopeptidase from human T cells
8. Prolyl endopeptidase from Flavobacterium meningosepticum: Cloning and sequencing of the enzyme gene
9. Characterization of a prolyl endopeptidase from Flavobacterium meningosepticum: Complete sequence and localization of the active-site serine
10. Prolyl endopeptidase from Aeromonas hydrophila: Cloning, sequencing, and expression of the enzyme gene, and characterization of the expressed enzyme
11. Purification and characterization of human brain prolyl endopeptidase
12. Peptidyldiazomethanes: A novel mechanism of interaction with prolyl endopeptidase
13. pH dependent mechanism in the catalysis of prolyl endopeptidase from pig muscle
14. Prolyl oligopeptidase: An unusual β-propeller domain regulates proteolysis
15. A simplified method of quantitating proteins using the Biuret and phenol reagents
16. Measurement of protein using bicinchoninic acid
17. The implications of multiple forms of phenylalanine hydroxylase in phenylketonuria and related diseases of phenylalanine metabolism
18. Relationship between the multiple forms of rat hepatic phenylalanine hydroxylase and degree of phosphorylation
19. Cleavage of structural proteins during assembly of the head of structural bacteriophage T4
20. Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining
21. Modified colorimetric ninhydrin methods for peptidase assay
22. Statistical analysis of enzyme kinetic data
23. Inhibition of rabbit brain prolyl endopeptidase by N-benzyloxycarbonyl prolyl-Prolinal: A transition state aldehyde inhibitor
24. An Endo acting proline-specific oligopeptidase from Treponema denticola ATCC 35405: Evidence of hydrolysis of human bioactive peptides
25. Post-proline cleaving enzyme (prolyl endopeptidase) from bovine brain
26. Post-proline cleaving enzyme: Purification of this endopeptidase by affinity chromatography
27. Purification and properties of a prolyl endopeptidase from rabbit brain
28. Studies of prolyl endopeptidase from Shakashimeji (Lyophyllum cinerascens): Purification and enzymatic properties
29. Porcine liver succinyltrialanine p-nitroanilide hydrolytic enzyme: Its purification and characterization as a post proline cleaving enzyme
30. Characterisation of proline endopeptidase from rat brain
31. Partial purification and characterization of post-proline cleaving enzyme: Enzymatic inactivation of neurohypophyseal hormones by kidney preparations of various species
32. Unusual specificity of prolyl oligopeptidase and the different reactivities of its two forms towards charged substrates
33. The interaction of α2-macroglobulin with proteinases: Characteristics of specificity of reaction and a hypothesis concerning its molecular mechanism
34. Porcine muscle prolyl endopeptidase and its endogenous substrates
35. Angiotensin II receptor antagonists
36. Substance P enhancement of passive and active conditioning in mice
37. Clinical applications of thyrotropin-releasing hormone
38. Purification and properties of a bovine brain thyrotropin-releasing-factor deamidase: A post-proline cleaving enzyme of limited specificity
39. Evaluation of the role of prolyl endopeptidase and pyroglutamyl peptidase in the metabolism of LH-RH and TRH in brain
40. Lower serum prolyl endopeptidase enzyme activity in major depression: Further evidence that peptidases play a role in the pathophysiology of depression
41. Degradation of neurotensin by rabbit brain endo-oligopeptidase A and endo-oligopeptidase B (proline endopeptidase)