Regio-selective detection of dynamic structure of transmembrane α-helices as revealed from 13C NMR spectra of [3-13C]Ala-labeled bacteriorhodopsin in the presence of Mn2+ ion

Biophysical Journal

Volumn 81, Issue 1, 2001, Pages 425-434

  Tuzi, Satoru ^a   Hasegawa, Jun ^a   Kawaminami, Ruriko ^a   Naito, Akira ^a   Saitô, Hazime ^a  

^a UNIVERSITY OF HYOGO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; BACTERIORHODOPSIN; MANGANESE;

ALPHA HELIX; ARTICLE; BACTERIUM DETECTION; CARBON NUCLEAR MAGNETIC RESONANCE; CONFORMATIONAL TRANSITION; COVALENT BOND; ELECTRON SPIN RESONANCE; HYDROPHOBICITY; NONHUMAN; PROTEIN CONFORMATION; PROTEIN LOCALIZATION; SITE DIRECTED MUTAGENESIS; X RAY DIFFRACTION;

BACTERIA (MICROORGANISMS);

EID: 0034950588     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)75710-4     Document Type: Article

References (50)

1. NMR chemical shift and electronic structure
2. Proton relaxation times in paramagnetic solutions
3. 1H NMR spectra of proteins as probes of surface structure and dynamics
4. Solid-state nuclear magnetic resonance characterization of gramicidin channel structure
5. High-resolution x-ray structure of an early intermediate in the bacteriorhodopsin photocycle
6. Lipid patches in membrane protein oligomers: Crystal structure of the bacteriorhodopsin-lipid complex
7. Thermal motions and function of bacteriorhodopsin in purple membranes: Effects of temperature and hydration studied by neutron scattering
8. Function and picosecond dynamics of bacteriorhodopsin in purple membrane at different lipidation and hydration
9. Calcium and magnesium binding in native and structurally perturbed purple membrane
10. Electron-crystallographic refinement of the structure of bacteriorhodopsin
11. Bacteriorhodopsin: The functional details of a molecular machine are being resolved
12. Distance estimates from paramagnetic enhancements of nuclear relaxation in linear and flexible model peptides
13. 13C NMR
14. 13C]Val-labeled transmembrane peptides of bacteriorhodopsin in lipid bilayers: Insertion, rigid-body motions, and local conformational fluctuations at ambient temperature
15. Surface of bacteriorhodopsin revealed by high-resolution electron crystallography
16. Proton translocation mechanism and energetics in the light-driven pump bacteriorhodopsin
17. Mechanism of ion transport across membranes. Bacteriorhodopsin as a prototype for proton pumps
18. Atomic resolution structures of bacteriorhodopsin photocycle intermediates: The role of discrete water molecules in the function of this light-driven ion pump
19. Coupling photoisomerization of retinal to directional transport in bacteriorhodopsin
20. Structure of bacteriorhodopsin at 1.55 Å resolution
21. Structural changes in bacteriorhodopsin during ion transport at 2 angstrom resolution
22. NMR structural studies of membrane proteins
23. From femtoseconds to biology: Mechanism of bacteriorhodopsin's light-driven proton pump
24. High sensitivity electron diffraction analysis. A study of divalent cation binding to purple membrane
25. The structure of bacteriorhodopsin at 3.0 Å resolution based on electron crystallography: Implication of the charge distribution
26. Properties of Asp212-Asn bacteriorhodopsin suggest that Asp212 and Asp85 both participate in a counterion and proton acceptor complex near the Schiff base
27. Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane
28. A synthetic medium for extremely halophilic bacteria
29. Rhodopsin and bacteriorhodopsin: Structure-function relationships
30. Lipidic cubic phase crystallization of bacteriorhodopsin and cryotrapping of intermediates: Towards resolving a revolving photocycle
31. X-ray structure of bacteriorhodopsin at 2.5 angstroms from microcrystals grown in lipidic cubic phases
32. Constraints on the flexibility of bacteriorhodopsin's carboxyl-terminal tail at the purple membrane surface
33. a's of asp-85 and glu-204 forms part of the reprotonation switch of bacteriorhodopsin
34. Transverse relaxation of dipolar coupled spin systems under rf irradiation: Detecting motions in solid
35. 13C chemical shifts: A new means of conformational characterization as obtained by high-resolution solid-state NMR
36. High-resolution solid-state NMR studies of synthetic and biological macromolecules
37. Empirical vs. nonempirical evaluation of secondary structure of fibrous and membrane proteins
38. 13C NMR
39. Structural alterations for proton translocation in the M state of wild-type bacteriorhodopsin
40. Relaxation processes in a system of two spins
41. Bacteriorhodopsin and related pigments of Halobacteria
42. 82 may function as an information mediator
43. 13C]Ala-labeled bacteriorhodopsin
44. 13C NMR
45. 13C nuclear magnetic resonance
46. 13C NMR
47. 13C NMR
48. 13C NMR
49. 13C NMR