Cylindrical diameters method for calibrating section thickness in serial electron microscopy

Journal of Microscopy

Volumn 202, Issue 3, 2001, Pages 468-472

  Fiala, J C ^a   Harris, K M ^a  

^a Boston University   (United States)

Author keywords

Alignment; Mitochondria; Serial sections; Stereology; Three dimensional reconstruction; Ultrathin sections

Indexed keywords

CYLINDRICAL OBJECTS; MITOCHONDRIAS; SECTION THICKNESS; SERIAL SECTION; STEREOLOGY; THREE-DIMENSIONAL RECONSTRUCTION; ULTRA-THIN; ULTRATHIN SECTION;

ACCURACY; ARTICLE; CALIBRATION; ELECTRON MICROSCOPY; MITOCHONDRION; PRIORITY JOURNAL; THICKNESS;

EID: 0034949692     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.2001.00926.x     Document Type: Article

References (8)

1. Comparison of methods for the estimation of the thickness of ultrathin tissue sections
2. Microwave fixation of rat hippocampal slices
3. Extending unbiased stereology of brain ultrastructure to three-dimensional volumes
4. Study of dendritic spines by serial electron microscopy and three-dimensional reconstructions
5. Preservation of neuronal ultrastructure in hippocampal slices using rapid microwave-enhanced fixation
6. Slices have more synapses than perfusion-fixed hippocampus from both young and mature rats
7. Thin sections. I. A study of section thickness and physical distortion produced during microtomy
8. Measurement of section thickness