Detection of fluorescence dye-labeled proteins in 2-D gels using an Arthur™ 1442 multiwavelength fluoroimager

BioTechniques

Volumn 31, Issue 1, 2001, Pages 146-149

  Herick, K ^a   Jackson, P ^a   Wersch, G ^a   Burkovski, A ^a  

^a PerkinElmer Life Sciences   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COST EFFECTIVENESS; DYES; ELECTROPHORESIS; FLUORESCENCE; GELS; NETWORK PROTOCOLS; PH EFFECTS; SILVER;

ISOELECTRIC FOCUSING (IEF);

PROTEINS;

BACTERIAL PROTEIN; BROMOBIMANE; CYSTEINE; FLUORESCENT DYE; POLYACRYLAMIDE GEL;

ARTICLE; CHEMICAL LABELING; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; ISOELECTRIC FOCUSING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DETERMINATION; SILVER STAINING; TECHNIQUE;

EID: 0034947845     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01311pf02     Document Type: Article

References (9)

1. Proteome analysis of rat hepatic stellate cells
2. Background-free, high sensitivity staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels using a luminescent ruthenium complex
3. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
4. Two-dimensional electrophoretic analysis of Corynebacterium glutamicum membrane fraction and surface proteins
5. Mapping and identification of Corynebacterium glutamicum proteins by two-dimensional gel electrophoresis and microsequencing
6. Application of SYPRO Orange and SYPRO Red protein gel stains
7. SYPRO Orange and SYPRO Red protein gel stains: One-step fluorescent staining of denaturing gels for detection of nanogram levels of protein
8. Difference gel electrophoresis: A single gel method for detecting changes in protein extracts
9. Two-dimensional polyacrylamide gel electrophoresis of proteins labeled with the fluorophore monobromobimane prior to first-dimensional isoelectric focusing; imaging of the fluorescent protein spot patterns using a cooled charge-coupled device