Use of degenerate oligonucleotide primed PCR (DOP-PCR) for the genotyping of low-concentration DNA samples

Journal of Molecular Medicine

Volumn 79, Issue 5-6, 2001, Pages 329-332

  Barbaux, Sandrine ^a   Poirier, Odette ^a   Cambien, François ^a  

^a INSERM   (France)

Author keywords

Degenerate oligonucleotide primed amplification (DOP PCR); DNA; Genotyping; Single nucleotide polymorphism (SNP); Whole genome amplification

Indexed keywords

DNA; OLIGONUCLEOTIDE;

ARTICLE; GENE AMPLIFICATION; GENE DELETION; GENE INSERTION; GENOTYPE; HUMAN; HUMAN CELL; POLYMERASE CHAIN REACTION; SINGLE NUCLEOTIDE POLYMORPHISM;

AUTORADIOGRAPHY; DNA; GENOTYPE; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; SAMPLE SIZE; SENSITIVITY AND SPECIFICITY; SEQUENCE DELETION;

EID: 0034944566     PISSN: 09462716     EISSN: None     Source Type: Journal    
DOI: 10.1007/s001090100214     Document Type: Article

References (9)

1. Fluorescence in situ hybridisation of YAC clones after Alu-PCR amplification
2. Whole genome amplification from a single cell: Implications for genetic analysis
3. Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer
4. Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes
5. Whole genome amplification using a degenerate oligonucleotide primer allows hundreds of genotypes to be performed on less than one nanogram of genomic DNA
6. Degenerate oligonucleotide-primed PCR (DOP-PCR): Evaluation of its reliability for screening genetic alterations in neoplasia
7. Optimizing DOP-PCR for universal amplification of small DNA samples in comparative genomic hybridisation
8. Detailed chromosomal and molecular genetic analysis of single cells by whole genome amplification and comparative genomic hybridisation
9. Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele-specific oligonucleotide probes