An invasive cleavage assay for direct quantitation of specific RNAs

Nature Biotechnology

Volumn 19, Issue 7, 2001, Pages 673-676

  Eis, Peggy S ^a   Olson, Marilyn C ^a   Takova, Tsetska ^a   Curtis, Michelle L ^a   Olson, Sarah M ^a   Vener, Tatiana I ^a   Ip, Hon S ^a   Vedvik, Kevin L ^a   Bartholomay, Christian T ^a   Allawi, Hatim T ^a   Ma, Wu Po ^a   Hall, Jeff G ^a   Morin, Michelle D ^a   Rushmore, Tom H ^b   Lyamichev, Victor I ^a   Kwiatkowski, Robert W ^a  

^a Third Wave Technologies Inc   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; DRUG PRODUCTS; ENZYMES; FLUORESCENCE; ISOTHERMS; RNA;

GENE EXPRESSION;

GENETIC ENGINEERING;

NUCLEASE; RNA;

ACCURACY; ARTICLE; BIOENGINEERING; CELL LYSATE; ENERGY TRANSFER; GENE EXPRESSION; MEDICAL GENETICS; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RNA ANALYSIS; RNA CLEAVAGE; RNA PROCESSING; RNA TRANSCRIPTION;

BASE SEQUENCE; BIOTECHNOLOGY; HIV; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; RNA; RNA, MESSENGER; SEQUENCE HOMOLOGY, NUCLEIC ACID; SPECTROMETRY, FLUORESCENCE;

EID: 0034943523     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/90290     Document Type: Article

References (20)

1. Plasma RNA viral load predicts the rate of CD4 T cell decline and death in HIV-2-infected patients in West Africa
2. Exploring the new world of the genome with DNA microarrays
3. Protein diversity from alternative splicing: A challenge for bioinformatics and post-genome biology
4. Polymorphism identification and quantitative detection of genomic DNA by invasive cleavage of oligonucleotide probes
5. Real time kinetics of restriction endonuclease cleavage monitored by fluorescence resonance energy transfer
6. Xplore mRNA assays for the quantification of IL-1 beta and TNF-alpha mRNA in lipopolysaccharide-induced mouse macrophages
7. Experimental and theoretical analysis of the invasive signal amplification reaction
8. The kinetics of oligonucleotide replacements
9. Sensitive detection of DNA polymorphisms by the serial invasive signal amplification reaction
10. RNA template-dependent 5′ nuclease activity of Thermus aquaticus and Thermus thermophilus DNA polymerases
11. Clinical, genetic, and pharmacogenetic applications of the invader assay
12. P450 superfamily: Update on new sequences, gene mapping, accession numbers and nomenclature
13. The thermodynamic advantage of DNA oligonucleotide 'stacking hybridization' reactions: Energetics of a DNA nick
14. Mapping of RNA accessible sites by extension of random oligonucleotide libraries with reverse transcriptase
15. Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family
16. Dexamethasone regulates IL-1 beta and TNF-alpha-induced interleukin-8 production in human bone marrow stromal and osteoblast-like cells
17. RNA processing and the evolution of eukaryotes
18. A comparison of eubacterial and archaeal structure-specific 5′-exonucleases