Mapping the receptor binding regions of human chorionic gonadotropin (hCG) using disulfide peptides of its β-subunit: Possible involvement of the disulfide bonds Cys9-Cys57 and Cys23-Cys72 in receptor binding of the hormone

Journal of Peptide Research

Volumn 58, Issue 1, 2001, Pages 17-26

  Mishra, A K ^a   Mahale, S D ^a   Sankaranarayana Iyer, K ^a  

^a Institute for Research in Reproduction India   (India)

Author keywords

Directed synthesis of disulfide peptides; Disulfide peptides of the subunit; Human chorionic gonadotropin; Radioreceptor assay; Receptor binding regions; Role of disulfide bonds

Indexed keywords

CHORIONIC GONADOTROPIN; CHORIONIC GONADOTROPIN BETA SUBUNIT; CHORIONIC GONADOTROPIN RECEPTOR; LUTEINIZING HORMONE RECEPTOR;

ARTICLE; BETA CHAIN; BINDING SITE; COMPETITIVE INHIBITION; CORPUS LUTEUM; COVALENT BOND; CROSS LINKING; CRYSTAL STRUCTURE; DISULFIDE BOND; DRUG RECEPTOR BINDING; HORMONE RECEPTOR INTERACTION; ISOTOPE LABELING; PEPTIDE MAPPING; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN TERTIARY STRUCTURE; RADIORECEPTOR ASSAY; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; BINDING SITES; BINDING, COMPETITIVE; CHORIONIC GONADOTROPIN; CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN; CYSTEINE; DISULFIDES; HUMANS; INHIBITORY CONCENTRATION 50; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; RECEPTORS, LH;

OVIS ARIES;

EID: 0034936291     PISSN: 1397002X     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3011.2001.00866.x     Document Type: Article

References (35)

1. Studies on the reduction and reoxidation of the disulfide bonds of the α and β subunits of human choriogonadotropin
2. Elimination of disulfide bonds affects assembly and secretion of the human chorionic gonadotropin β-subunit
3. Immunological properties of the β-subunit of human chorionic gonadotropin
4. Immunological properties of the β-subunit of human chorionic gonadotropin. I. Effects of chemical and enzymatic modification
5. Crystal structure of human chorionic gonadotropin
6. Structure of human chorionic gonadotropin at 2.6 Å resolution from MAD analysis of selenomethionyl protein
7. Receptor binding regions of hLH and hCG β-subunit: Structural and functional properties
8. A receptor binding site in the region 81-95 of the β subunit of the human luteinizing hormone (LH) and chorionic gonadotropin (hCG)
9. Studies on the delineation of the hormone-specific antigenic determinants of human follicle-stimulating hormone
10. Identification of bioneutralization epitopes of human follicle stimulating hormone in the regions 31-52 and 66-75 of its β-subunit
11. Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides
12. Tissue sulphhydryl groups
13. Formation of disulfide bonds in synthetic peptides and proteins
14. Efficient approach to synthesis of two-chain asymmetric cysteine analogs of receptor-binding region of transforming growth factor-α
15. 3-Nitro-2-pyridine sulfenyl group for protection and activation of the thiol function of cysteine
16. Comparative evaluation of different methods for disulfide bond formation in synthesis of the HIV-2 antigenic determinant
17. Sensitive quantitative analysis of disulfide bonds in polypeptide and proteins
18. Development of an LH receptor assay capable of measuring serum LH/CG in a wide variety of species
19. Protein and cell membrane iodinations with a sparingly soluble chloramide 1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril
20. Delineation of subunit and receptor contact sites by site-directed mutagenesis of hCG-β
21. Search for peptide immunogens of the β-subunit of human chorionic gonadotropin (hCG) capable of eliciting hormone specific and neutralizing antisera: Identification of an undecapeptide eliciting hCG-specific antisera
22. Model of human chorionic gonadotropin and lutropin receptor interaction that explains signal transduction of the glycoprotein hormones
23. The groove between the alpha- and beta-subunits of hormones with lutropin (LH) activity appears to contact LH receptor, and its conformation is changed during hormone binding
24. Comparative studies of mammalian glycoprotein hormones
25. Role of the β (93-100) determinant loop sequence in receptor binding and biological activity of human luteinizing hormone and chorionic gonadotropin
26. Conversion of human chorio gonadotropin into a follitropin by protein engineering
27. Role of the invariant aspartic acid 99 of human choriogonadotropin β in receptor binding and biological activity
28. Contributions of arginine-43 and -94 of human chorio gonadotropin β to receptor binding and activation as determined by oligonucleotide-based mutagenesis
29. A single amino acid residue replacement in the β-subunit of human chorionic gonadotropin results in the loss of biological activity
30. Mutagenesis of the 'determinant loop' region of human chorionic gonadotropin β
31. Co-evolution of ligand-receptor pairs
32. Peptide mapping of intersubunit and receptor interactions of human choriogonadotropin
33. Amino/carboxy-terminus deletion mutants of choriogonadotropin β
34. The lutropin β-subunit N-terminus facilitates subunit combination by offsetting the inhibitory effects of residues needed for LH activity
35. A receptor binding region in human choriogonadotropin/lutropin β-subunit