Multiplex PCR combining transgene and S-allele control primers to simultaneously confirm cultivar identity and transformation in apple

Plant Cell Reports

Volumn 20, Issue 4, 2001, Pages 349-353

  Broothaerts, W ^a   Wiersma, P A ^a   Lane, W D ^a  

^a Better3Fruit nv ^*  (Belgium)

Author keywords

Duplex PCR; Malus x domestica; Self incompatibility gene; Transformation; Trueness to type

Indexed keywords

ALLELE; CULTIVAR IDENTIFICATION; GENETIC CONSERVATION; GENETIC TRANSFORMATION; PLANT BIOTECHNOLOGY; PLANT BREEDING; PLANT REGENERATION; POLYMERASE CHAIN REACTION; TRANSGENE;

CELLS; GENETIC ENGINEERING; GREENHOUSE EFFECT;

GENETIC TRANSFORMATION;

PLANTS (BOTANY);

MALUS X DOMESTICA;

EID: 0034936049     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002990100321     Document Type: Article

References (15)

1. Factors affecting the transformation of 'Marshall McIntosh' apple by Agrobacterium tumefaciens
2. pBINPLUS: An improved plant transformation vector based on pBIN19
3. Differentiation of closely related but biologically distinct cherry isolates of Prunus necrotic ringspot virus by polymerase chain reaction
4. A molecular method for S-allele identification in apple based on allele-specific PCR
5. Weitere Untersuchungen über die Befruchtungsverhältnisse der Apfel- und Birnesorten
6. TriPrimer-PCR method: Rapid and reliable detection of transgenes in transgenic rice plants
7. Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers
8. Screening of transgenic plants by multiplex PCR
9. Investigation of Agrobacterium-mediated transformation of apple using green fluorescent protein: High transient expression and low stable transformation suggests factors other than T-DNA transfer are rate-limiting
10. Regeneration of adventitious shoots in process of genetic transformation
11. Molecular detection and identification of intimin alleles in pathogenic Escherichia coli by multiplex PCR
12. Multiplex polymerase chain reaction for the simultaneous detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Chlamidia psittaci in respiratory samples
13. Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation
14. Use of the multi-allelic self-incompatibility gene in apple to assess homozygosity in shoots obtained through haploid induction