Molecular characterization of the actin-encoding gene and the use of its promoter for a dominant selection system in the methylotrophic yeast Hansenula polymorpha

Applied Microbiology and Biotechnology

Volumn 55, Issue 6, 2001, Pages 734-741

  Kang, H A ^a   Hong, W K ^a   Sohn, J H ^a   Choi, E S ^a   Rhee, S K ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; DNA; HYGROMYCIN B; MESSENGER RNA; RECOMBINANT PROTEIN;

YEAST;

ANTIBIOTIC RESISTANCE; ARTICLE; DNA SEQUENCE; GENETIC MANIPULATION; HANSENULA POLYMORPHA; INTRON; MOLECULAR CLONING; NONHUMAN; PLASMID; PROMOTER REGION; RNA SPLICING; SACCHAROMYCES CEREVISIAE; TATA BOX; YEAST;

ACTINS; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; FUNGAL PROTEINS; GENES, FUNGAL; HYGROMYCIN B; INTRONS; MOLECULAR SEQUENCE DATA; ORGANOPLATINUM COMPOUNDS; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PICHIA; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; RNA SPLICING; RNA, FUNGAL; RNA, MESSENGER; SELECTION (GENETICS); TRANSFORMATION, GENETIC;

EID: 0034923138     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530100605     Document Type: Article

References (34)

1. An unusual actin-encoding gene in Physarum polycephalum
2. Introns and protein revolution - An analysis of the exon/intron organization of actin genes
3. Efficient selection of hygromycin B-resistant Yarrowia lipolytica transformants
4. Dominant selection system for use in Cryptococcus neoformans
5. Kluyveromyces lactis maintains Saccharomyces cerevisiae intron-encoded splicing single
6. The single-copy actin gene of Phytophthora megasperma encodes a protein considerably diverged from any other known actin
7. The major promoter element of rRNA transcription in yeast lies 2 kb upstream
8. Structure of a split yeast gene: Complete nucleotide of the actin gene in Saccharomyces cerevisiae
9. Stable multicopy integration of vector sequences in Hansenula polymorpha
10. Recombinant Hansenula polymorpha as a biocatalyst: Coexpression of the spinach glycolate oxidase (GO) and the S. cerevisiae catalase T (CTT1) gene
11. Plasmid-encoded hygromycin B resistance: The sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae
12. Nucleotide sequences of the rabbit alpha-smooth-muscle and beta-non-muscle actin mRNA
13. Yeast/E. coli shuttle vectors with multiple unique restriction sites
14. Production of recombinant proteins by methylotrophic yeasts
15. A rapid efficient method for isolating DNA from yeast
16. Simultaneous expression of the S and L surface antigens of hepatitis B and formation of mixed particles in the methylotrophic yeast, Hansenula polymorpha
17. Identification and developmental expression of a smooth-muscle gamma-actin in postmeiotic male germ cells of mice
18. Sequence of the Candida albicans gene encoding actin
19. Structure, organization and promoter expression of the actin-encoding gene in Trichoderma reesei
20. A singly intronless actin gene in the fission yeast Schizosaccharomyces pombe: Nucleotide sequence and transcripts formed in homologous and heterologous yeast
21. Nuclear pre-mRNA splicing in the fission yeast Schizosaccharomyces pombe strictly requires an intron-contained, conserved sequence element
22. DNA sequences required for yeast actin gene transcription do not include conserved CCAAT motifs
23. Actin
24. Transformation of filamentous fungi based on hygromycin B and phleomycin resistance markers
25. Recombinant DNA in filamentous fungi: Progress and prospects
26. A dominant selection system designed for copy-number-controlled gene integration in Hansenula polymorpha DL-1
27. A family of telomere-associated autonomous replicating sequences and their functions in targeted recombination in Hansenula polymorpha DL-1
28. Nucleotide sequence and expression of the human skeletal alpha-actin gene: Evolution of functional regulatory domains
29. Peroxisome biogenesis in the yeast Hansenula polymorpha is controlled by a complex set of interacting gene products
30. At least six different actins are expressed in a higher mammal: An analysis based on the amino acid sequence of the amino-terminal tryptic peptide
31. The amino acid sequence of Physarum actin
32. Structural and phylogenetic analysis of the actin gene from the yeast Phaffia rhodozyma
33. Nuclear pre-mRNA splicing in yeast