Synthesis of α-gal epitopes (Galα1-3Galβ1-4GlcNAc-R) on human tumor cells by recombinant α1,3galactosyltransferase produced in Pichia pastoris

Glycobiology

Volumn 11, Issue 7, 2001, Pages 577-586

  Chen, Zhao Chun ^a   Tanemura, Masahiro ^a   Galili, Uri ^b  

^a RUSH MEDICAL COLLEGE   (United States)

^b RUSH UNIVERSITY   (United States)

Author keywords

Anti Gal; Pichia pastoris; Recombinant 1,3galactosyltransferase; Tumor vaccines; gal epitope

Indexed keywords

ALPHA 1,3 FUCOSYLTRANSFERASE; AMINO ACID; ANTIBODY; AUTOANTIGEN; CARBOHYDRATE DERIVATIVE; COMPLEMENTARY DNA; CYTOPLASM PROTEIN; DETERGENT; EPITOPE; FC RECEPTOR; GALACTOSE; GALACTOSYLTRANSFERASE; GLYCOCONJUGATE; GLYCOSYLTRANSFERASE; HISTIDINE DERIVATIVE; IMIDAZOLE; IMMUNOGLOBULIN G; MEMBRANE PROTEIN; METHANOL; N ACETYLGALACTOSAMINE; N ACETYLGLUCOSAMINE; NICKEL; RECOMBINANT ENZYME; SEPHAROSE; SIALIDASE; SIALYLTRANSFERASE; TUMOR ANTIGEN; TUMOR VACCINE; UNINDEXED DRUG; URIDINE DIPHOSPHATE GALACTOSE;

ACUTE GRANULOCYTIC LEUKEMIA; ANTIGEN PRESENTING CELL; ANTINEOPLASTIC ACTIVITY; ARTICLE; BINDING AFFINITY; CANCER PATIENT; CARBOHYDRATE ANALYSIS; CELL MEMBRANE; CONTROLLED STUDY; CULTURE MEDIUM; DRUG SYNTHESIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SYNTHESIS; HUMAN; HUMAN CELL; IN VIVO STUDY; LEUKEMIA CELL LINE; MARMOSET; NONHUMAN; OVARY CARCINOMA; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; SOLUBILITY; TUMOR CELL LINE; YEAST;

MAMMALIA; MARMOSETS; PICHIA PASTORIS; PLATYRRHINI;

EID: 0034920584     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/11.7.577     Document Type: Article

References (35)

1. Enzymatic synthesis of blood group related pentaglycosyl ceramide by an α-galactosyltransferase
2. Two α-3-D galactosyltransferases in rabbit stomach mucosa with different acceptor substrate specificities
3. An α-D-galactosyltransferase in Ehrlich ascites tumor cells: Biosynthesis and characterization of a trisaccharide (α-D-galacto(1-3)-N-acetyllactosamine)
4. Biosynthesis of terminal Galα1-3Gal81-4GlcNAc-R oligosaccharide sequence on glycoconjugates: Purification and acceptor specificity of a UDP-Gal: N-acetyllactosamine α1, 3galactosyltransferase
5. Conversion of a Golgi apparatus sialyltransferase to a secretory protein by replacement of the NH2-terminal signal anchor with a signal peptide
6. Cardiac xenografts between primate species provide evidence of the α-galactosyl determinant in hyperacute rejection
7. Interaction of the natural anti-Gal antibody with α-galactosyl epitopes: A major obstacle for xenotransplantation in humans
8. Synthesis of the α-galactosyl (Galα1-3Gal-β1-4GlcNAc-R) epitope on humans cells by recombinant primate α1,3galactosyltransferase expressed in E. coli
9. Natural anti-Gal antibody as a universal augmenter of autologous tumor vaccine immunogenicity
10. Gene sequences suggest inactivation of α1,3 galactosyltransferase in catarrhines after the divergence of apes from monkeys
11. A unique natural human IgG antibody with anti-α-galactosyl specificity
12. Human natural anti-α-galactosyl IgG. II. The specific recognition of α(1-3)-linked galactose residues
13. Evolutionary relationship between the anti-Gal antibody and the Galα1-3Gal epitope in primates
14. Man, apes, and Old World monkeys differ from other mammals in the expression of α-galactosyl epitopes on nucleated cells
15. A sensitive assay for measuring α-gal epitope expression on cells by a monoclonal anti-Gal antibody
16. Heterologous expression of an engineered truncated form of human Lewis fucosyltransferase (Fuc-TIII) by the methylotrophic yeast Pichia pastoris
17. Identification of carbohydrate structures which bind human anti-porcine antibodies: Implication for discordant xenografting in man
18. The asparagine-linked oligosaccharides on bovine fetuin. Structural analysis of N-glycanase-released oligosaccharides by 500-megahertz 1H NMR spectroscopy
19. Cloning of a novel constitutively expressed pectate lyase gene pelB from Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of the gene product expressed in Pichia pastoris
20. Defining the minimal size of catalytically active primate α1, 3 galactosyltransferase: Structure function studies on the recombinant truncated enzyme
21. Synthesis of α-gal epitopes on influenza virus vaccines by recombinant α1,3galactosyltransferase enables the formation of immune complexes with the natural anti-Gal antibody
22. Bovine α1-3-galactosyltransferase: Isolation and characterization of a cDNA clone. Identification of homologous sequences in human genomic DNA
23. α1-3-Galactosyltransferase: The use of recombinant enzyme for the synthesis of α-galactosylated glycoconjugates
24. Isolation of a cDNA encoding murine UDP galactose: βD-galactosyl-1,4-N-acetyl-D-glucosaminide α1,3-galactosyltransferase: Expression cloning by gene transfer
25. Frameshift and nonsense mutations in a human genomic sequence homologous to a murine UDP-Gal α-D-Galβ(1,4)-D-GlcNAc α(1,3)galactosyltransferase cDNA
26. Synthesis of α-galactosyl epitopes by recombinant α1,3galactosyltransferase for opsonization of human tumor cell vaccines by anti-galactose
27. Increased immunogenicity of tumor vaccines complexed with anti-Gal: Studies in knock out mice for α1,3galactosyltransferase
28. Expression of functional soluble forms of human β-1,4-galactosyltransferase I, α-2,6-sialyltransferase, and α-1,3-fucosyltransferase VI in the methylotrophic yeast Pichia pastoris
29. Anti-pig IgM antibodies in human serum react predominantly with Gal α1-3Gal epitopes
30. Transfer and expression of a murine UDP-Gal: α-D-Gal α1, 3-galactosyltransferase gene in transfected Chinese Hamster Ovary cells. Competition reactions between the α1,3-galactosyltransferase and the α2,3-sialyltransferase
31. Porcine cartilage transplants in cynomolgus monkey: III. Transplantation of α-galactosidase treated porcine cartilage
32. Differential expression of α-gal epitopes (Gal α1-3Galβ1-4GlcNAc-R) on pig and mouse organs
33. The differential expression of Gal α1→3Galβ1→4GlcNAc-R residues on mammalian secreted N-glycosylated glycoproteins
34. Expression of functional mouse 5-HT5A serotonin receptor in the methylotrophic yeast Pichia pastoris: Pharmacological characterization and localization
35. 4 and B4 isolated from Bandeiraea simplicifolia