Preparation and use of specific antibodies to the β-I and β-II subunits of gonadotropic hormone from Fundulus heteroclitus pituitary

General and Comparative Endocrinology

Volumn 123, Issue 2, 2001, Pages 203-209

  Calman, Bruce ^a   Lin, Yu Wai Peter ^b   Wallace, Robin A ^a,c  

^a UNIVERSITY OF FLORIDA   (United States)

^b BARRY UNIVERSITY   (United States)

^c UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

Author keywords

Antibodies to GtH subunits I and II; Gonadotropin (GtH); Immunoblot; Immunocytochemistry; Killifish; Mummichog (Fundulus heteroclitus); Pituitary gland; Teleost

Indexed keywords

GONADOTROPIN; GONADOTROPIN I BETA SUBUNIT, FUNDULUS; GONADOTROPIN II BETA SUBUNIT, FUNDULUS; PEPTIDE FRAGMENT; ANTIBODY; BRAIN PROTEIN; PROTEIN SUBUNIT;

AMINO ACID SEQUENCE; ANIMAL; ANIMAL MODEL; ARTICLE; CHEMISTRY; CYPRINODONTIFORMES; HISTOLOGY; HYPOPHYSIS; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; METABOLISM; ANIMAL TISSUE; ANTIBODY LABELING; CONTROLLED STUDY; FISH; FUNDULUS HETEROCLITUS; HYPOPHYSIS CELL; HYPOPHYSIS PARS DISTALIS; IMMUNOCYTOCHEMISTRY; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANIMAL; FUNDULIDAE; GONADOTROPINS, PITUITARY; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; MODELS, ANIMAL; PEPTIDE FRAGMENTS; PITUITARY GLAND; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0034917299     PISSN: 00166480     EISSN: None     Source Type: Journal    
DOI: 10.1006/gcen.2001.7671     Document Type: Article

References (46)

1. The pituitary gland: Anatomy and histophysiology
2. Immunocytochemical demonstration of pituitary cell types in the teleost Poecilia latipinna by light and electron microscopy
3. The ovary of Fundulus heteroclitus
4. The normal menstrual cycle
5. Pattern of vitellogenesis and follicle maturational competence during the ovarian follicular cycle of Fundulus heteroclitus
6. Isolation of gonadotropin subunits and evidence for two distinct gonadotropins in Atlantic croaker (Micropogonias undulatus)
7. Localization of prolactin within the pituitary of a cyprinodont fish, Fundulus heteroclitus (Linnaeus), by specific fluorescent antiovine prolactin globulin
8. The development of monoclonal antibodies against salmon (Oncorhynchus kisutch and O. Keta) pituitary hormones and their immunohistochemical identification
9. Protein G-horseradish peroxidase based method for light-microscope immunocytochemistry: Application to the pituitary gland of the killifish, Fundulus heteroclitus
10. Oocyte maturation in the mummichog (Fundulus heteroclitus): Effects of steroids on germinal vesicle breakdown in intact follicles in vitro
11. Molecular cloning and sequence analysis of striped bass (Morone saxatilis) gonadotropin-I and gonadotropin-II subunits
12. Comparison of semilunar cycles of spawning activity in Fundulus grandis and F. Heteroclitus held under constant laboratory conditions
13. Reproductive cycling in female Fundulus heteroclitus
14. Semilunar follicular cycle of an intertidal fish: The Fundulus model
15. Specific antibodies against synthetic peptides of salmonid GTH I and II β subunits
16. cDNA cloning of the beta subunit of teleost thyrotropin
17. Oogenesis in Fundulus heteroclitus. VI. Establishment and verification of conditions for vitellogenin incorporation by oocytes in vitro
18. Isolation and characterization of two distinct gonadotropins, GtHI and GtHII, from bonito (Katsuwonus plelamis) pituitary glands
19. Cleavage of structural proteins during assembly of the head of the bacteriophage T4
20. Fundulus hetroclitus gonadotropins. 4. Cloning and sequencing of gonaotropic hormone (GTH) α-subunit
21. Fundulus heteroclitus gonadotropin(s) I. Homologous bioassay using oocyte maturation and steroid production by isolated ovarian follicles
22. Fundulus heteroclitus gonadotropin(s) 2. Year-round husbandry of animals with active pituitaries and active follicles
23. Fundulus heteroclitus gonadotropins. 3. Cloning and sequencing of gonadotropic hormone (GTH) I and II β-subunits using the polymerase chain reaction
24. The pituitary gland of Fundulus
25. Differential production and regulation of gonadotropins (GTH I and GTH II) in the pituitary gland of rainbow trout, Oncorhynchus mykiss, during ovarian development
26. Salmon pituitary gonadotrophs. I. Distinct cellular distributions of two gonadotropins, GTH I and GTH II
27. Purification and characterization of gonadotropin I and II from pituitary glands of tuna (Thunnus obesus)
28. Immobilon PVDF transfer membrane: A new membrane substrate for Western blotting of proteins
29. Cloning and sequence of thyrotropin β-subunit of a teleost fish the eel (Anguilla anguilla)
30. The life cycle of the brain-pituitary-gonad-axis in teleosts
31. Gametogenesis in Fundulus heteroclitus
32. Cellular aspects of oocyte growth in teleosts
33. Cytological changes in the teleost pituitary gland associated with the reproductive cycle
34. Fixation of ejaculated spermatozoa for electron microscopy
35. Development of salmon GTH I and GTH II radioimmunoassays
36. Isolation and characterization of two coho salmon gonadotropins, GTH I and GTH II
37. Purification of gonadotropins (PmGTH I and II) from red seabream (Pagrus major) and development of a homologous radioimmunoassay for PmGTH II
38. Estuarine and intertidal teleosts
39. Cloning and sequencing of the cDNA encoding the pituitary gonadotropin II β-subunit of yellowfin porgy (Acanthopagrus latus)
40. Properties of common carp gonadotropin I and gonadotropin II
41. The cellular origin of pituitary gonadotropins in teleosts
42. Oogenesis in Fundulus heteroclitus I. Preliminary observations on oocyte maturation in vivo and in vitro
43. Oogenesis in Fundulus heteroclitus II. The transition from vitellogenesis into maturation
44. Histochemical and immunocytochemical identification of the pituitary cell types in three sciaenid fishes: Atlantic croaker (Micropogonias undulatus), spotted seatrout, (Cynoscion nebulosus), and red drum (Sciaenops ocellatus)
45. Molecular cloning of the cDNAs encoding two gonadotropin β subunits (GTH-Iβ and-IIβ) from the goldfish
46. Duality of gonadotropin in a primitive teleost, Japanese eel (Anguilla japonica)