Site-directed mutagenesis and phylogenetic comparisons of the Escherichia coli Tus protein: DNA-protein interactions alone can not account for Tus activity

Molecular Genetics and Genomics

Volumn 265, Issue 6, 2001, Pages 941-953

  Henderson, T A ^a   Nilles, A F ^a   Valjavec Gratian, M ^b   Hill, T M ^a  

^a UNIVERSITY OF NORTH DAKOTA SCHOOL OF MEDICINE AND HEALTH SCIENCES   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

DNA replication; Escherichia coli; Replication arrest; Site directed mutagenesis; Tus

Indexed keywords

BACTERIAL PROTEIN; MUTANT PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BINDING AFFINITY; DNA REPLICATION; ESCHERICHIA COLI; GENE EXPRESSION; KLEBSIELLA; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION; PROTEIN PROTEIN INTERACTION; SALMONELLA; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; YERSINIA;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; DNA REPLICATION; DNA, BACTERIAL; DNA-BINDING PROTEINS; ENTEROBACTERIACEAE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; KLEBSIELLA; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHYLOGENY; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; PROTEUS MIRABILIS; SALMONELLA ENTERITIDIS; SALMONELLA TYPHIMURIUM; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SERRATIA MARCESCENS; YERSINIA ENTEROCOLITICA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; KLEBSIELLA; SALMONELLA; YERSINIA;

EID: 0034915312     PISSN: 16174615     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380100501     Document Type: Article

References (30)

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2. E. coli replication terminator protein impedes Simian Virus 40 (SV40) DNA replication fork movement and SV40 large tumor antigen helicase activity in vitro at a prokaryotic terminus sequence
3. Termination of DNA replication of bacterial and plasmid chromosomes
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5. Biophysical characteristics of Tus, the replication arrest protein of Escherichia coli
6. The importance of repairing stalled replication forks
7. Using modified nucleotides to map the DNA determinants of the Tus-TerB complex, the protein-DNA interaction associated with termination of replication in Escherichia coli
8. Equilibrium, kinetic, and footprinting studies of the Tus-Ter protein-DNA interaction
9. Tight regulation, modulation, and high-level expression by vectors containing the arabinose P13AD promoter
10. Differential inhibition of the DNA translocation and DNA unwinding activities of DNA helicases by the Escherichia coli Tus protein
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12. Features of the chromosomal terminus
13. Escherichia coli Tus protein acts to arrest the progression of DNA replication forks in vitro
14. Identification of the DNA sequence from the E. coli terminus region that halts replication forks
15. Structure of a replication-terminator protein complexed with DNA
16. The replication terminator protein of the gram-positive bacterium Bacillus subtilis functions as a polar contrahelicase in gram-negative Escherichia coli
17. The replication terminator protein of E. coli is a DNA sequence-specific contra-helicase
18. Site-specific deletions of chromosomally located DNA segments with a multimer resolution system of broad-host-range plasmid RP4
19. Recombinational repair of DNA damage in Escherichia coli and bacteriophage λ
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21. Mutagenesis by incorporation of a phosphorylated oligo during PCR
22. Interaction of the Escherichia coli replication terminator protein (Tus) with DNA: A model derived from DNA-binding studies of mutant proteins by surface plasmon resonance
23. Role of PriA in replication fork reactivation in Escherichia coli
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26. Isolation and characterization of mutants of Tus, the replication arrest protein of Escherichia coli
27. Mutations in the Escherichia coli Tus protein define a domain positioned close to the DNA in the Tus-Ter complex
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29. Bound lac repressor differentially inhibits the unwinding reactions catalyzed by DNA helicases
30. The Bacillus subtilis replication terminator system functions in Escherichia coli