Purification and characterization of Δ1-pyrroline-5-carboxylate reductase isoenzymes, indicating differential distribution in spinach (Spinacia oleracea L.) leaves

Plant and Cell Physiology

Volumn 42, Issue 7, 2001, Pages 742-750

  Murahama, M ^a   Yoshida, T ^a   Hayashi, F ^a   Ichino, T ^a   Sanada, Y ^a   Wada, K ^a  

^a KANAZAWA UNIVERSITY   (Japan)

Author keywords

Chloroplast; Isoenzemes; Partial amino acid sequence; Proline biosynthesis; Spinach (Spinacia oleracea); Water stress; 1 Pyrroline 5 carboxylate (P5C)

Indexed keywords

AMMONIUM SULFATE; ANION EXCHANGE CHROMATOGRAPHY; BIOCHEMICAL PATHWAY; BIOSYNTHESIS; CHLOROPLAST; CHROMATOGRAPHY; COMPLEMENTARY DNA; ENZYME PURIFICATION; ENZYME SUBUNIT; FRACTIONATION; ISOENZYME; MAGNESIUM ION; MOLECULAR WEIGHT; PYRROLINE 5 CARBOXYLATE REDUCTASE; REACTION MECHANISM; SEQUENCE HOMOLOGY;

EMBRYOPHYTA; SPINACIA OLERACEA;

EID: 0034913745     PISSN: 00320781     EISSN: None     Source Type: Journal    
DOI: 10.1093/pcp/pce093     Document Type: Article

References (51)

1. Distinct cellular and organismic responses to salt stress
2. Temporal and spatial distribution of ferredoxin isoproteins in tomato fruit
3. Photosynthetic and heterotrophic ferredoxin isoproteins are colocalized in fruit plastids of tomato
4. Chlorophyll fluorescence as a possible tool for salinity tolerance screening in barley (Hordeum vulgare L.)
5. Proline accumulation in higher plants: A redox buffer?
6. Method to obtain a chlorophyll-free preparation of intact mitochondria from spinach leaves
7. Solute accumulation in tobacco cells adapted to NaCl
8. Plant productivity and environment
9. Pyrroline-5-carboxylate reductase in soybean nodules: Isolation/partial primary structure/evidence for isozymes
10. Physiological and genetic responses of bacteria to osmotic stress
11. ABA and the transcriptional control of CAM induction during salt stress in the common ice plant
12. 1-pyrroline-5-carboxylate reductase was isolated by functional complementation in Escherichia coli and is found to be osmoregulated
13. Proline biosynthesis and osmoregulation in plants
14. Purification, characterization and cDNA sequence of glucose-6-phosphate dehydrogenase from potato (Solanum tuberosum L.)
15. Solutes contributing to osmotic adjustment in cultured plant cells adapted to water stress
16. Biosynthesis of 3-dimethylsulfoniopropionate in Wollastonia biflora (L.) DC. Evidence that S-methylmethionine is an intermediate
17. Induction of Crassulacean acid metabolism in Mesembryanthemum cystallinum by high salinity: Mass increase and de novo synthesis of PEP-carboxylase
18. 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants
19. Purification to homogeneity of pyrroline-5-carboxylate reductase of barley
20. 1-pyrroline-5-carboxylate reductase
21. Multiple forms of alcohol dehydrogenase in Saccharomyces cerevisiae. 1. Physiological control of ADH-2 and properties of ADH-2 and ADH-4
22. 1-Pyrroline-5-carboxylate reductase from baker's yeast. Further purification by activity chromatography with 5′ AMP-sepharose 4B
23. Drought and salt tolerance: Toward understanding and application
24. Purified human erythrocyte pyrroline-5-carboxylate reductase. Preferential oxidation of NADPH
25. Properties and analysis of a stable derivative of pyrroline-5-carboxylic acid for use in metabolic studies
26. Pyrroline-5-carboxylic acid reductase from soybean leaves
27. Molecular cloning and expression of an otolith matrix protein cDNA from the rainbow trout, Oncorhynchus mykiss
28. An improved method for isolating chloroplasts retaining their outer membranes
29. A simple method for determination of intramolecular amino acid sequence of unpurified protein
30. 1-pyrroline-5-carboxylate reductase from tobacco leaves
31. Pinitol, a compatible solute in Mesembry-anthemum crystallinum L.?
32. Pyrroline-5-carboxylate reductase is in pea (Pisum sativum L.) leaf chloroplasts
33. Proline dehydrogenase and pyrroline-5-carboxylate reductase from pumpkin cotyledons
34. Metabolic changes associated with adaptation of plant cells to water stress
35. 1-pyrroline-5-carboxylate reductase from Escherichia coli
36. Novel light-dark change of proline levels in halophyte (Mesembryanthemum crystallinum L.) and glycophytes (Hordeum vulgare L. and Triticum aestivum L.) leaves and roots under salt stress
37. 1-pyrroline-5-carboxylate synthetase in Arabidopsis thaliana
38. Tricine-sodium dodecyl sulfate-polyacylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
39. Purification and characterization of rat rens pyrroline-5-carboxylate reductase
40. Hydroxyl radical scavenging activity of compatible solutes
41. 1-pyrroline-5-carboxylate reductase in root/nodule and leaf of soybean
42. Stress protection of transgenic tobacco by production of the osmolyte mannitol
43. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
44. A survey of plants for leaf peroxisomes
45. 1-pyrroline-5-carboxylate reductase in proline-accumulating cell suspension cultures of Mesembryanthemum nodiflorum and other halophytes
46. Organic acids as sources of drought-induced proline synthesis in field bean plants, Vicia faba L.
47. Osmoregulation of a pyrroline-5-caboxylate reductase gene in Arabidopsis thaliana
48. Complete nucleotide sequence of the chloroplast genome from the green alga Chlorella vulgaris: The existence of genes possibly involved in chloroplast division
49. Regulation of proline biosynthesis in kiwifruit buds with and without hydrogen cyanamide treatment
50. Molecular cloning and evidence for osmoregulation of the Δ1-pyrroline-5-carboxylate reductase (pro C) gene in pea (Pisum sativum L.)
51. 1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress