Comparative analysis of monoclonal antibody based enzyme immunoassay, modified genome electrophoresis & electron microscopy procedures for rotavirus diagnosis from faecal specimens

Indian Journal of Medical Research

Volumn 113, Issue MAR., 2001, Pages 78-82

  Saravanan, P ^a   Ananthan, S ^a   Sundaram, A ^a  

^a Dr ALM Post Grad Inst Basic Med ^*  (India)

Author keywords

Acute diarrhoea; Diagnosis; ELISA; EM; PAGE; Rotavirus

Indexed keywords

MONOCLONAL ANTIBDY YO 156; MONOCLONAL ANTIBODY; UNCLASSIFIED DRUG;

ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; ELECTRON MICROSCOPY; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; FECES CULTURE; GASTROENTERITIS; GEL ELECTROPHORESIS; HUMAN; INFANT; MAJOR CLINICAL STUDY; METHODOLOGY; NEWBORN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRESCHOOL CHILD; ROTAVIRUS; SENSITIVITY AND SPECIFICITY; STANDARDIZATION; ULTRACENTRIFUGATION; VIRUS DETECTION; VIRUS INFECTION;

ANTIBODIES, MONOCLONAL; CHILD; DIARRHEA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FECES; GENOME; HUMANS; MICROSCOPY, ELECTRON; ROTAVIRUS; ROTAVIRUS INFECTIONS; SENSITIVITY AND SPECIFICITY; ULTRACENTRIFUGATION;

EID: 0034909326     PISSN: 09715916     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Efficacy of live, attenuated, human rotavirus vaccine 89-12 in infants: A randomised placebo-controlled trial
2. Rotavirus epidemiology in Vellore, south India: Group, subgroup, serotype and electropherotype
3. Viruses in acute gastroenteritis in children in Pune, India
4. Epidemiological and clinical characteristics of acute diarrhoea in children due to human rotavirus
5. Prevalence of rotavirus diarrhoea among hospitalized children in Pune, India
6. Characterization of rotaviruses from children with acute diarrhoea in Delhi
7. Rotavirus detection by dot blot hybridization assay using a non-radioactive synthetic oligodeoxynucleotide probe
8. Latex immunoassay for rapid detection of rotavirus
9. Electron microscopy procedure influences detection of rotaviruses
10. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels
11. Detection of rotavirus with a new polyclonal antibody enzyme immunoassay (Rotazyme II) and a commercial latex agglutination test (Rotalex): Comparison with a monoclonal antibody enzyme immunoassay
12. Accuracy of rotavirus diagnosis: Modified genome electrophoresis versus electron microscopy
13. Evaluation of different methods (ELISA, IF, EM, PAGE) for the diagnosis of rotavirus infection in piglets
14. Comparison of five enzyme immunoassays, electron microscopy, and latex agglutination for detection of rotavirus in fecal specimens
15. Comparison of seven kits for detection of rotavirus in faecal specimens with a sensitive, specific enzyme immunoassay
16. Validity of an enzyme-linked immunosorbent assay with serotype - Specific monoclonal antibodies for serotyping human rotavirus in stool specimens
17. Analysis of human rotavirus serotypes in children with acute diarrhoea in Chennai by monoclonal antibody based ELISA
18. Section mounting
19. Choice of reference assay for the detection of rotavirus in fecal specimens: Electron microscopy versus enzyme immunoassay
20. Isolation, characterization and serial propagation of a bovine group C rotavirus in a monkey kidney cell line (MA 104)
21. The value of latent class analysis in medical diagnosis
22. Random effects models in latent class analysis for evaluating accuracy of diagnostic tests
23. Approximate binomial confidence limits
24. Comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children
25. Comparative evaluation of a commercial enzyme-linked immunoassay and solid-phase immune electron microscopy for rotavirus detection in stool specimens
26. Comparison of a new rapid test (Testpack rotavirus) with standard enzyme immunoassay and electron microscopy for the detection of rotavirus in symptomatic hospitalized children
27. Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay
28. Detection of human group C rotaviruses by an enzyme-linked immunosorbent assay using monoclonal antibodies
29. Atypical rotavirus genomic patterns identified by polyacrylamide gel electrophoresis
30. Rotaviruses