Asking probing questions: Can fluorescent in situ hybridization identify and localise aquatic hyphomycetes on leaf litter?

International Review of Hydrobiology

Volumn 86, Issue 4-5, 2001, Pages 429-438

  McArthur, Fiona A ^a   Baerlocher, Mark O ^a   MacLean, Natalie A B ^b   Hiltz, Megan D ^c   Bärlocher, Felix ^a  

^a MOUNT ALLISON UNIVERSITY   (Canada)

^b CROSS CANCER INSTITUTE   (Canada)

^c UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Aquatic hyphomycetes; Heliscus lugdunensis; Rhodamine 123; Ribosomal stains; rRNA

Indexed keywords

FLUORESCENCE ANALYSIS; FUNGUS DETECTION; GENETIC STRAIN; LEAF LITTER; RHODAMINE 123; RIBOSOME RNA; RNA 16S; SPECIFICITY; STAINING;

AQUATIC ENVIRONMENT; FLUORESCENCE; FUNGUS; IDENTIFICATION METHOD; LEAF LITTER;

EID: 0034898517     PISSN: 14342944     EISSN: None     Source Type: Journal    
DOI: 10.1002/1522-2632(200107)86:4/5<429::AID-IROH429>3.0.CO;2-9     Document Type: Article

References (40)

1. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
2. The oligonucleotide probe database
3. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology
4. Identification in situ and phylogeny of uncultured bacterial endosymbionts
5. In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes
6. Fluorescently-primed in situ PCR in arbuscular mycorrhizas
7. Dynamics of the fungal populations on leaves in a stream
8. Conidium production from leaves and needles in four streams
9. The role of fungi in the nutrition of stream invertebrates
10. Development of monoclonal antibody-based immunoassay for the detection and quantification of anguillospora longissima colonizing leaf material
11. Use of immunoassays for the study of natural assemblages of aquatic hyphomycetes
12. The study of microorganisms in situ: Progress and problems
13. Rate and mode differences between nuclear and mitochondrial small subunit rRNA genes in mushrooms
14. Molecular tools for the identification of ectomycorrhizal fungi - Taxon-specific oligonucleotide probes for suilloid fungi
15. The spatial distribution of fungi on decomposing alder leaves in a freshwater stream
16. Cell-wall degrading enzymes of aquatic hyphomycetes: A review
17. Multiple sequence alignment with hierarchical clustering
18. Multiple colors by flourescence in sitar hybridization using ratio-labeled DNA probes create a molecular karyo-type
19. Phylogenetic stains: Ribosomal RNA-based probes for the identification of single cells
20. Single-cell identification using fluorescently labeled, ribosomal RNA-specific probes
21. Community analysis of biofilters using fluorescence in situ hybridization including a new probe for the Xanthomonas branch of the class Proteobacteria
22. Fungal biomass, production and sporulation associated with particulate organic matter in streams
23. Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells
24. Decomposition of autumn-shed beech leaves in a springbrook and its significance for the fauna
25. Fluorescent probes for living cells
26. Isolation of DNA from fungal mycelia and single spores
27. PCR primers that allow intergeneric differentiation of Ascomycetes and their application to Verticillium sp.
28. Growth and reproduction in aquatic hyphomycetes
29. Food selection in three leaf-shredding stream invertebrates
30. Conidia and classification of the nectrioid fungi
31. Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives
32. Comparison of three techniques for the study of aquatic hyphomycete communities
33. Fungi and bacteria associated with leaves during processing in a woodland stream
34. Interactions with invertebrates
35. The teleomorphs of water-borne Hyphomycetes from fresh water
36. Anamorph-teleomorph relationships
37. Selective recovery of 16S rRNA sequences from natural microbial communities in the form of cDNA
38. Amplification and direct sequencing of ribosomal RNA genes for phylogenetics