Combining DAPI and SYBR Green II for the enumeration of total bacterial numbers in aquatic sediments

International Review of Hydrobiology

Volumn 86, Issue 4-5, 2001, Pages 453-465

  Griebler, Christian ^a,c   Mindl, Birgit ^b   Slezak, Doris ^a  

^a ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

^b UNIVERSITY OF INNSBRUCK   (Austria)

^c UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Biofilm porewater ratios; Enumeration; Epifluorescence; Sediment bacteria; SYBR Green II

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; AQUATIC ENVIRONMENT; BACTERIAL GROWTH; BIOFILM; CORRELATION ANALYSIS; HABITAT; PARTICLE; SEDIMENT;

ABUNDANCE ESTIMATION; AQUATIC ENVIRONMENT; BACTERIUM; SEDIMENT;

EID: 0034897495     PISSN: 14342944     EISSN: None     Source Type: Journal    
DOI: 10.1002/1522-2632(200107)86:4/5<453::AID-IROH453>3.0.CO;2-L     Document Type: Article

References (56)

1. Bacterial productivity and microbial biomass in tropical mangrove sediments
2. Detection of active yeast cells (Saccharomyces cerevisiae) in frozen dough sections
3. Hyporheic biofilms - A potential food source for interstitial animals
4. Growth and metabolic flexibility in groundwater bacteria
5. Comparison of acridine orange, acriflavine and bisbenzinide for enumeration of bacteria in clear and humic waters
6. Bacterial biomass, metabolic state, and activity in stream sediments: Relation to environmental variables and multiple assay comparison
7. 2 to groundwater
8. Structure and function of biofilms
9. Enhanced detection of bacteria in natural environments by fluorochrome staining of DNA
10. Microbial biofilms
11. Bacteria in intertidal sediments: Factors related to their distribution
12. Direct counts of aquatic bacteria by a modified epifluorescence technique
13. Relationship between bacteria and grain surfaces in intertidal sediments
14. A method for the quantitative estimation of bacteria from mangrove sediments
15. Comparison of homogenization and ultrasonication as techniques in extracting attached sedimentary bacteria
16. Enumeration of sandy sediment bacteria: Search for optimal protocol
17. Spatial distribution and respiration of bacteria in stream-bed sediments
18. Direct methods and biomass estimation
19. Sessile bacteria: An important component of the microbial population in small mountain streams
20. Enumeration and morphological characterization of bacteria indigenous to subsurface environments
21. Ein neuer ansatz zur bestimmung der bakteriellen abundanz in bach- und flußsedimenten. Methodenvergleich und effizienzbestimmung. - 32
22. Biofilme in Grundwasser-Ökosystemen
23. Distribution patterns of attached and unattached bacteria in pristine and contaminated shallow aquifers studied with an in situ sediment exposure microcosm (ISSEM)
24. Effect of organic contamination upon microbial distribution and heterotrophic uptake in a Cape Cod, Mass., aquifer
25. Use of Nucleopore filters for counting bacteria by fluorescence microscopy
26. Some observations on direct counts of freshwater bacteria obtained with a fluorescence microscope
27. A microbiological study of sediments from the Cumbrian lakes
28. Use of fluorochromes for direct enumeration of total bacteria in environmental samples: Past and present
29. Microbial communities in the saturated groundwater environment. I. Methods of isolation and characterization of heterotrophic bacteria
30. Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems
31. Organic matter in gravel streams (Project Ritrodat-Lunz)
32. The DNA distribution in planktonic bacteria stained with TOTO or TO-PRO
33. Attached microbial communities in rivers
34. Groundwater microbiology: Subsurface ecosystem processes
35. Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1, and Pico Green for flow cytometric analysis of marine prokaryotes
36. Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I
37. Physiological assessment of bacteria using fluorochromes
38. Fluctuations and interactions of bacterial activity in sandy beach sediments and overlying waters
39. Mikrobielle besiedelung und produktion
40. Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria
41. The use of DAPI for identifying and counting aquatic microflora
42. Direct counts of bacteria in the sediments of a North Carolina salt marsh
43. Solutions to problems in enumerating sediment bacteria by direct counts
44. The ecology of sediment bacteria in lakes and comparisons with other aquatic ecosystems
45. The use of fluorescence microscopy for counting soil microorganisms
46. Particle size, water stable aggregates, and bacterial populations in lake sediments
47. Microscopic enumeration of attached marine bacteria of seawater, marine sediment, fecal matter and kelp blade samples following pyrophosphate and ultrasound treatments
48. Improved sample preparation for enumeration of aggregated aquatic substrate bacteria
49. Heterotrophic microorganisms
50. Determination of microbial cell numbers in subsurface samples
51. Utility of green fluorescent nucleic acid dyes and aluminium oxide membrane filters for rapid epifluorescence enumeration of soil and sediment bacteria
52. A new method for fluorescence staining of bacterial populations on membrane filters