Laser-assisted microdissection of membrane-mounted paraffin sections for polymerase chain reaction analysis: Identification of cell populations using immunohistochemistry and in situ hybridization

Journal of Molecular Diagnostics

Volumn 3, Issue 3, 2001, Pages 105-110

  Gjerdrum, Lise Mette ^a   Lielpetere, Ilze ^b   Rasmussen, Lars Melholt ^a   Bendix, Knud ^a   Hamilton Dutoit, Stephen ^a  

^a AARHUS UNIVERSITY HOSPITAL   (Denmark)

^b PAULS STRADINS CLINICAL UNIVERSITY HOSPITAL   (Latvia)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN; CYTOKERATIN; DNA; GENE PRODUCT; NUCLEIC ACID; PARAFFIN; PROTEOME;

ANTIGEN RETRIEVAL; ARTICLE; CELL POPULATION; CLINICAL PROTOCOL; CONTROLLED STUDY; EPSTEIN BARR VIRUS; GENETIC ANALYSIS; HEAD AND NECK CARCINOMA; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; LASER; LOW TEMPERATURE PROCEDURES; MEMBRANE; OPTICS; POLYMERASE CHAIN REACTION; STANDARD; TARGET CELL; TISSUE SECTION;

HUMAN HERPESVIRUS 4;

EID: 0034893965     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1525-1578(10)60659-9     Document Type: Article

References (13)

1. Microbeam MOMeNT: Non-contact laser microdissection of membrane mounted native tissue
2. Distinct cytogenetic alterations in squamous intraepithelial lesions of the cervix revealed by laser-assisted microdissection and comparative genomic hybridization
3. Gene expression profiles of laser-captured adjacent neuronal subtypes
4. Laser capture microdissection
5. Identification of expressed genes by laser-mediated manipulation of single cells
6. PT-PCR from archival single cells is a suitable method to analyze specific gene expression
7. Immuno-LCM: Laser capture microdissection of immunostained frozen sections for mRNA analysis
8. Genomic analysis of single cells from human basal cell cancer using laser-assisted capture microscopy
9. Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains
10. Detection of t(2;5)(p23;q35) translocation by reverse transcriptase polymerase chain reaction and in situ hybridization in CD30-positive primary cutaneous lymphoma and lymphomatoid papulosis
11. Epstein-Barr virus types 1 and 2 differ in their EBNA-3A, EBNA-3B and EBNA-3C genes
12. Epstein-Barr virus infection and the pathogenesis of nasopharyngeal carcinoma: Viral gene expression, tumour cell phenotype, and the role of the lymphoid stroma
13. Influence of histochemical and immunohistochemical stains on polymerase chain reaction