Active transport of an antibiotic rifamycin derivative by the outer-membrane protein FhuA

Structure

Volumn 9, Issue 8, 2001, Pages 707-716

  Ferguson, Andrew D ^a,b,c   Ködding, Jiri ^a   Walker, Georg ^d   Bös, Christoph ^d   Coulton, James W ^c   Diederichs, Kay ^a   Braun, Volkmar ^d   Welte, Wolfram ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^c MCGILL UNIVERSITY   (Canada)

^d UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Active transport; Antibiotic; Crystal structure; Integral membrane protein; Structure based drug design; TonB dependent transporter

Indexed keywords

CGP 4832; FERRIC HYDROXAMATE UPTAKE PROTEIN COMPONENT A; FERRICHROME; GRISEIN; LIGAND; OUTER MEMBRANE PROTEIN; RIFAMYCIN DERIVATIVE; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL MEMBRANE; BINDING COMPETITION; BINDING SITE; DRUG PROTEIN BINDING; DRUG TRANSPORT; ESCHERICHIA COLI; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ALLOSTERIC SITE; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; BINDING SITES; BIOLOGICAL TRANSPORT; BIOLOGICAL TRANSPORT, ACTIVE; CELL MEMBRANE; CRYSTALLOGRAPHY, X-RAY; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FERRICHROME; LIGANDS; MEMBRANE PROTEINS; MODELS, MOLECULAR; MUTATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, VIRUS; RIFAMYCINS; SPECTROMETRY, FLUORESCENCE; TIME FACTORS; TRYPTOPHAN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0034886699     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(01)00631-1     Document Type: Article

References (34)

1. Structure and function of the porin channel
2. The proton motive force drives outer membrane transport of cobalamin in Escherichia coli
3. Bacterial iron transport: Mechanisms, genetics, and regulation
4. Siderophore-mediated iron transport: Crystal structure of FhuA with bound lipopolysaccharide
5. Transmembrane signalling across the ligand-gated FhuA receptor: Crystal structures of free and ferrichrome-bound states reveal allosteric changes
6. Iron uptake and molecular recognition in Pseudomonas putida: Receptor mapping with ferrichrome and its biomimetic analogs
7. Crystal structure of the antibiotic albomycin in complex with the outer membrane transporter FhuA
8. CGP 4832, a semisynthetic rifamycin derivative highly active against some Gram-negative bacteria
9. Highly efficient uptake of a rifampicin derivative via the FhuA-TonB-dependent uptake route in Escherichia coli
10. Genetic control of hydroxamate-mediated iron uptake in Escherichia coli
11. Oligomeric states and siderophore binding of ligand-gated FhuA protein that forms channels across Escherichia coli outer membranes
12. Specific in vivo labelling of cell surface-exposed protein loops: Reactive cysteines in the predicted gating loop mark a ferrichrome binding site and a ligand-induced conformational change of the Escherichia coli. FhuA protein
13. Transport across the outer membrane of Escherichia coli K12 via the FhuA receptor is regulated by the TonB protein of the cytoplasmic membrane
14. Ligand-induced conformational change in the ferrichrome-iron receptor of Escherichia coli K-12
15. Cell envelope signalling in Escherichia coli: Ligand binding to the ferrichrome-iron receptor FhuA promotes interaction with the energy-transducing protein TonB
16. In vivo evidence for FhuA outer membrane receptor interaction with the TonB inner membrane protein of Escherichia coli
17. Crystal structure of the outer membrane active transporter FepA from Escherichia coli
18. Site-directed disulfide bonding reveals an interaction site between energy-coupling protein TonB and BtuB, the outer membrane cobalamin transporter
19. Substrate-induced exposure of an energy-coupling motif of a membrane transporter
20. The β-barrel domain of FhuAΔ5-160 is sufficient for TonB-dependent FhuA activities of Escherichia coli
21. FhuA, a transporter of the Escherichia coli outer membrane is converted into a channel upon the binding of bacteriophage T5
22. Reconstitution of FhuA, an Escherichia coli outer membrane protein, into liposomes. Binding of phage T5 to FhuA triggers the transfer of DNA into the proteoliposomes
23. Identification of a new site for ferrichrome transport by comparison of the FhuA proteins of Escherichia coli, Salmonella paratyphi B, Salmonella typhimurium, and Pantoea agglomerans
24. Molekulare wirkungsweise des antibioticums rifampicin
25. Structural basis for sugar translocation through maltoporin channels at 3.1 Å resolution
26. Structure of ScrY, the sucrose-specific porin from Salmonella typhimurium, and its complex with sucrose at 2.4 Å resolution
27. Evaluation of single crystal X-ray diffraction data from a position sensitive detector
28. Improved methods for building protein models in electron density maps and the location of errors in these models
29. Crystallography & NMR system: A new software suite for macromolecular structure determination
30. Genetic insertion and exposure of a reporter epitope in the ferrichrome-iron receptor of Escherichia coli K-12
31. An internal affinity-tag for purification and crystallization of the siderophore receptor FhuA, integral outer membrane protein from Escherichia coli K-12
32. Molscript: A program to produce both detailed and schematic plots of protein structures
33. Raster3D photorealistic molecular graphics